RNA测序分析为柑桔黄龙病菌的冷应激反应提供了见解。
RNA-seq analysis provides insights into cold stress responses of Xanthomonas citri pv. citri.
作者信息
Liao Jin-Xing, Li Kai-Huai, Wang Jin-Pei, Deng Jia-Ru, Liu Qiong-Guang, Chang Chang-Qing
机构信息
Integrative Microbiology Research Centre, South China Agricultural University, No. 483 Wushan Road, Tianhe, Guangzhou, 510642, People's Republic of China.
Department of Plant Pathology, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, No. 483 Wushan Road, Tianhe, Guangzhou, 510642, People's Republic of China.
出版信息
BMC Genomics. 2019 Nov 6;20(1):807. doi: 10.1186/s12864-019-6193-0.
BACKGROUND
Xanthomonas citri pv. citri (Xcc) is a citrus canker causing Gram-negative bacteria. Currently, little is known about the biological and molecular responses of Xcc to low temperatures.
RESULTS
Results depicted that low temperature significantly reduced growth and increased biofilm formation and unsaturated fatty acid (UFA) ratio in Xcc. At low temperature Xcc formed branching structured motility. Global transcriptome analysis revealed that low temperature modulates multiple signaling networks and essential cellular processes such as carbon, nitrogen and fatty acid metabolism in Xcc. Differential expression of genes associated with type IV pilus system and pathogenesis are important cellular adaptive responses of Xcc to cold stress.
CONCLUSIONS
Study provides clear insights into biological characteristics and genome-wide transcriptional analysis based molecular mechanism of Xcc in response to low temperature.
背景
柑橘溃疡病菌是一种导致柑橘溃疡病的革兰氏阴性细菌。目前，对于柑橘溃疡病菌对低温的生物学和分子反应了解甚少。
结果
结果表明，低温显著降低了柑橘溃疡病菌的生长，增加了生物膜形成和不饱和脂肪酸比例。在低温下，柑橘溃疡病菌形成分支结构的运动性。全局转录组分析表明，低温调节柑橘溃疡病菌中的多个信号网络以及碳、氮和脂肪酸代谢等重要细胞过程。与IV型菌毛系统和致病相关基因的差异表达是柑橘溃疡病菌对冷胁迫的重要细胞适应性反应。
结论
该研究为柑橘溃疡病菌响应低温的生物学特性及基于全基因组转录分析的分子机制提供了清晰的见解。
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