Gu X S, Song Z B, Jin Z W, Meng G R, Zhang C A, Yan D Y, Gu T, Yang S Q, He J R, Luo F J
Research Group of HFRS, Guang'an County, Sichuan.
Chin Med J (Engl). 1990 Jun;103(6):455-9.
A new strain of Hantaan virus (HTNV), GH716, was isolated from the peritoneal exudate cells (PEC) of a patient with severe hemorrhagic fever with renal syndrome (HFRS). The isolate was propagated in Vero E6 cells. At each passage the virus-infected cells were examined for HTNV with immunofluorescence technique using monoclonal antibodies (McAb) 25-1 and 84-1 against HTNV. From passage 5 on, fluorescent intensity tended to stabilize at (+++) and infectivity titer reached 10(5) TCID50/ml. Using 14 McAb to 76-118 and BI strains of HTNV, we found that strain GH716 was antigenically similar to strain 76-118 (Apodemus type) and different from strain R22 (Rattus type), suggesting that GH716 may fall into the Apodemus type of HTNV. This is the first isolation of an HTNV from human PEC collected on the tenth day of illness. The successful isolation of strain GH716 may provide an alternative source of obtaining HTNV during the later stages of HFRS.
从一名重症肾综合征出血热（HFRS）患者的腹腔渗出细胞（PEC）中分离出一种新型汉坦病毒（HTNV）毒株，即GH716。该毒株在Vero E6细胞中传代培养。在每一代传代过程中，使用针对HTNV的单克隆抗体（McAb）25 - 1和84 - 1，通过免疫荧光技术对病毒感染的细胞进行HTNV检测。从第5代开始，荧光强度趋于稳定在（+++），感染性滴度达到10(5) TCID50/ml。使用针对HTNV 76 - 118株和BI株的14种单克隆抗体，我们发现GH716毒株在抗原性上与76 - 118株（姬鼠型）相似，与R22株（大鼠型）不同，这表明GH716可能属于HTNV的姬鼠型。这是首次从发病第10天采集的人类PEC中分离出HTNV。GH716毒株的成功分离可能为在HFRS后期获得HTNV提供另一种来源。
