Zhang X K, Takashima I, Mori F, Hashimoto N
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University.
Microbiol Immunol. 1989;33(3):195-205. doi: 10.1111/j.1348-0421.1989.tb01513.x.
Two strains of hemorrhagic fever with renal syndrome (HFRS) virus from Rattus, SR-11 and KI-262, showed virtually identical antigenicity but differed from prototype strain Hantaan 76-118 (Apodemus origin) in a neutralization test. Wistar newborn rats inoculated intraperitoneally (i.p.) with SR-11, which was isolated from a laboratory rat associated with an outbreak of HFRS, developed clinical signs such as ataxia and limb paralysis and died at about 18 days after inoculation. The LD50 of SR-11 in 1-day-old rats was 10(1.2) focus-forming units (FFU). In contrast, the animals inoculated i.p. or intracerebrally with 10(4) FFU of KI-262, which was from a wild rat in a dumping-ground area--an enzootic focus where no human cases have been recorded--did not show any significant clinical signs. The susceptibility of rats to SR-11 fatal infection was age-dependent. Virus titers in brains, lungs, kidneys, and livers of the rats inoculated with SR-11 were significantly higher than those in the same organs of the animals infected with KI-262. Necrosis of neurons in the brain tissue occurred in the rats infected with SR-11, while it was mild in the animals infected with KI-262.
从褐家鼠分离出的两株肾综合征出血热（HFRS）病毒，SR - 11和KI - 262，在中和试验中显示出几乎相同的抗原性，但与原型株汉坦病毒76 - 118（姬鼠来源）不同。将从与HFRS暴发相关的实验大鼠中分离出的SR - 11腹腔内（i.p.）接种给新生Wistar大鼠后，大鼠出现共济失调和肢体麻痹等临床症状，并在接种后约18天死亡。SR - 11在1日龄大鼠中的半数致死剂量（LD50）为10（1.2）个焦点形成单位（FFU）。相比之下，将10（4）FFU的KI - 262腹腔内或脑内接种给大鼠，KI - 262是从垃圾场地区的野生大鼠分离得到的——这是一个没有人类病例记录的动物疫源地——大鼠未表现出任何明显的临床症状。大鼠对SR - 11致命感染的易感性具有年龄依赖性。接种SR - 11的大鼠脑、肺、肾和肝脏中的病毒滴度显著高于感染KI - 262的大鼠相同器官中的病毒滴度。感染SR - 11的大鼠脑组织中神经元发生坏死，而感染KI - 262的大鼠中坏死较轻。
