用于引起肠外感染的大肠杆菌的多位点可变数目串联重复序列分析
Multi-locus variable number tandem repeat analysis for Escherichia coli causing extraintestinal infections.
作者信息
Manges Amee R, Tellis Patricia A, Vincent Caroline, Lifeso Kimberley, Geneau Geneviève, Reid-Smith Richard J, Boerlin Patrick
机构信息
Department of Epidemiology, Biostatistics and Occupational Health, McGill University, 1020 Pine Avenue West, Montréal, Québec, Canada.
出版信息
J Microbiol Methods. 2009 Nov;79(2):211-3. doi: 10.1016/j.mimet.2009.09.006. Epub 2009 Sep 16.
Discriminatory genotyping methods for the analysis of Escherichia coli other than O157:H7 are necessary for public health-related activities. A new multi-locus variable number tandem repeat analysis protocol is presented; this method achieves an index of discrimination of 99.5% and is reproducible and valid when tested on a collection of 836 diverse E. coli.
对于与公共卫生相关的活动而言，用于分析除O157:H7之外的大肠杆菌的鉴别基因分型方法是必要的。本文提出了一种新的多位点可变数目串联重复序列分析方案；该方法的鉴别指数达到99.5%，并且在对836株不同的大肠杆菌进行测试时具有可重复性和有效性。
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