Doulah A H, Rohani A Haeri, Khaksari Haddad M, Motamedi F, Farbood Y, Badavi M, Malek M, Sarkaki A
Department of Biology, Sciences & Research Branch, Islamic Azad University (IAU), Poonak Squar, Ashrafi Isfehani High Way, Tehran, Iran.
Neurosci Lett. 2009 Nov 27;466(1):47-51. doi: 10.1016/j.neulet.2009.09.016. Epub 2009 Sep 17.
This study aimed to evaluate the peripheral administration of growth hormone (GH) on AD-like cognitive deficiency in NBM-lesioned rats induced by ibotenic acid (5 microg/microl, in each side). Forty-eight male Wistar rats (20-24 months old; weighing 330+/-30 g) randomly divided into six groups (n=8). The groups include control group, which were intact rats; n-L+GH group: non-lesioned rats with GH treatment (1mg/kg, 9.00 am, for 10 consecutive days); n-L+Veh group: non-lesioned rats with vehicle treatment; L group: NBM-lesioned rats; L+GH group: NBM-lesioned rats with GH treatment and L+Veh group: NBM-lesioned rats with same volume of vehicle treatment. Peripheral administration of GH in control had no effect on learning and memory, while in L+GH group produced a significant enhancement in spatial learning and memory comparing to L and L+Veh groups. The percent of time spent in goal quarter during probe trial has decreased significantly in L and L+Veh groups compared to n-L groups. While it has increased significantly in L+GH group compared to L and L+Veh groups. No significant difference in percent of time spent was seen between the control and n-L groups. The GH has known as a mediate that effect through IGF-1. As the IGF-1 itself is earlier shown to improve cognitive function it is likely that the observed effect of GH is mediated through release of IGF-1 from peripheral tissue into the circulation for further transport across the BBB. This mechanism may result in the improvement of learning and memory in rats with NBM lesion.
本研究旨在评估生长激素（GH）外周给药对鹅膏蕈氨酸（每侧5微克/微升）诱导的基底前脑内侧隔核（NBM）损伤大鼠类阿尔茨海默病（AD）样认知缺陷的影响。48只雄性Wistar大鼠（20 - 24月龄；体重330±30克）随机分为六组（n = 8）。这些组包括：对照组，即未损伤大鼠；n - L + GH组：接受GH治疗的未损伤大鼠（1毫克/千克，上午9点，连续10天）；n - L + Veh组：接受载体治疗的未损伤大鼠；L组：NBM损伤大鼠；L + GH组：接受GH治疗的NBM损伤大鼠；L + Veh组：接受等量载体治疗的NBM损伤大鼠。对照组外周给予GH对学习和记忆无影响，而与L组和L + Veh组相比，L + GH组在空间学习和记忆方面有显著增强。与n - L组相比，L组和L + Veh组在探索试验中在目标象限停留时间的百分比显著降低。而与L组和L + Veh组相比，L + GH组该百分比显著增加。对照组和n - L组在停留时间百分比上无显著差异。已知GH是通过胰岛素样生长因子-1（IGF - 1）发挥作用的介质。由于IGF - 1本身 earlier 已被证明可改善认知功能，所以GH观察到的效应可能是通过外周组织释放IGF - 1进入循环，进而穿过血脑屏障（BBB）来介导的。这种机制可能导致NBM损伤大鼠学习和记忆的改善。 （注：原文中“earlier”可能有误，推测可能是“earlier”，翻译为“先前” ）
