Malek Maryam, Sarkaki Alireza, Zahedi-Asl Saleh, Farbood Yaghoob, Rajaei Ziba
Isfahan University of Medical Sciences, School of Medicine, Department of Physiology, Isfahan, Iran.
Ahvaz Jundishapur University of Medical Sciences, Physiology Research Center, Ahvaz, Iran.
Arq Neuropsiquiatr. 2017 Jul;75(7):477-483. doi: 10.1590/0004-282X20170074.
In this study, we proposed that administration of hippocampal growth hormone in ageing animals with growth hormone deficiency can compensate long-term potentiation and synaptic plasticity in nucleus basalis magnocellularis (NBM)-lesioned rats. Aged male Wistar rats were randomly divided into six groups (seven in each) of sham-operated healthy rats (Cont); NBM-lesioned rats (L); NBM-lesioned rats and intrahippocampal injection of growth hormone vehicle (L + Veh); NBM-lesioned and intrahippocampal injection of growth hormone (10, 20 and 40 µg.2 µl-1) (L + GH). In vivo electrophysiological recording techniques were used to characterize maintenance of long-term potentiation at distinct times (1, 2, 3, 24 and 48 hours) after high-frequency stimulation. The population spike was enhanced significantly for about 48 hours following tetanic stimulation in rats treated with a dose-dependent growth hormone compared to the vehicle group (p < 0.05), possibly through neuronal plasticity and neurogenesis in affected areas.
在本研究中，我们提出，给生长激素缺乏的老龄动物注射海马生长激素，可以补偿基底前脑大细胞部（NBM）损伤大鼠的长时程增强和突触可塑性。将老龄雄性Wistar大鼠随机分为六组（每组七只）：假手术健康大鼠（对照组）；NBM损伤大鼠（损伤组）；NBM损伤大鼠并海马内注射生长激素溶媒（损伤+溶媒组）；NBM损伤并海马内注射生长激素（10、20和40μg·2μl-1）（损伤+生长激素组）。采用体内电生理记录技术，在高频刺激后的不同时间点（1、2、3、24和48小时）对长时程增强的维持情况进行表征。与溶媒组相比，给予剂量依赖性生长激素治疗的大鼠在强直刺激后约48小时，群体峰电位显著增强（p<0.05），这可能是通过受影响区域的神经元可塑性和神经发生实现的。
