Slavíková M, Lodinová-Zádníková R, Hanson L A, Adlerberth I, Carlsson B, Wold A E, Scanborg Edén C
Institute for the Care of Mother and Child, Prague.
Folia Microbiol (Praha). 1990;35(3):266-72. doi: 10.1007/BF02820494.
Dominant bacterial strains present in stool (with particular emphasis on E. coli strains) were examined in 4 groups of healthy infants: breast-fed and bottle-fed, colonized with E. coli O83, and control (non-colonized) breast-fed and bottle-fed newborns. The presence of fimbriae was examined by hemagglutination, the P-fimbriae-bearing strains were tested by the PPA latex test. In addition, adherence to cell line HT-29 and serotyping was performed in selected strains. The E. coli strain O83 was found to possess type 1 fimbriae. Fewer bacterial strains possessing type 1 fimbriae were found in E. coli O83-colonized infants (except the O83 serotype) than in control infants. The E. coli O83 strain colonized significantly better the breast-fed than the bottle-fed infants; its higher adherence activity was demonstrated even in cell line HT-29. Finally, colonization with E. coli O83 influenced the character of microbial intestinal flora: the frequency of positive E. coli isolates was significantly higher in colonized (both breast- and bottle-fed) than noncolonized infants.
对4组健康婴儿粪便中存在的主要细菌菌株（特别着重于大肠杆菌菌株）进行了检查：母乳喂养和人工喂养的婴儿，感染了大肠杆菌O83的婴儿，以及对照（未感染）的母乳喂养和人工喂养的新生儿。通过血凝反应检查菌毛的存在，通过PPA乳胶试验检测携带P菌毛的菌株。此外，对选定的菌株进行了对细胞系HT-29的黏附性检测和血清分型。发现大肠杆菌O83菌株具有1型菌毛。在感染大肠杆菌O83的婴儿（O83血清型除外）中，发现具有1型菌毛的细菌菌株比对照婴儿少。大肠杆菌O83菌株在母乳喂养婴儿中的定植明显优于人工喂养婴儿；即使在细胞系HT-29中也显示出其较高的黏附活性。最后，感染大肠杆菌O83影响了肠道微生物菌群的特征：在感染（母乳喂养和人工喂养）的婴儿中，大肠杆菌分离株呈阳性的频率明显高于未感染的婴儿。
