Kühn I, Tullus K, Möllby R
Infection. 1986 Jan-Feb;14(1):7-12. doi: 10.1007/BF01644802.
The aim of the present investigation was to study the intestinal colonization of Escherichia coli in newborn children, and to determine which strains become residential within the human intestine. The E. coli flora of 89 newborn children was studied by repeated sampling during their first 11 or 18 months of life. The E. coli isolates from the samples were subdivided into phenotypes by the aid of biochemical fingerprinting, a method which measures the kinetics of 24 selected biochemical tests as a tool for discriminating bacterial strains. It was found that E. coli strains colonizing children soon after birth persisted longer than strains colonizing them later. Especially those phenotypes which were defined as hospital strains persisted longer. Certain phenotypes were commonly found among the children, and these phenotypes were more persistent and more homogeneous than other phenotypes with respect to their pattern of biochemical activities. They might be specially adapted to colonize the human intestine. It was concluded that the generally long persistence of the first E. coli strains colonizing a newborn child indicates that the first case of bacterial colonization in children may be an event too important to be allowed to happen at random.
本研究的目的是研究新生儿中大肠杆菌的肠道定植情况，并确定哪些菌株会在人类肠道内定居。通过在89名新生儿生命的前11个月或18个月内反复采样，对其大肠杆菌菌群进行了研究。借助生化指纹图谱法将样本中的大肠杆菌分离株细分为不同的表型，该方法通过测量24项选定生化试验的动力学来鉴别细菌菌株。研究发现，出生后不久定植于儿童体内的大肠杆菌菌株比后来定植的菌株持续时间更长。特别是那些被定义为医院菌株的表型持续时间更长。某些表型在儿童中普遍存在，并且就其生化活性模式而言，这些表型比其他表型更持久、更具同质性。它们可能特别适应于在人类肠道内定植。研究得出结论，最初定植于新生儿的大肠杆菌菌株通常具有较长的持续时间，这表明儿童首次细菌定植可能是一个非常重要的事件，不应任其随机发生。
