Clinic for Avian, Reptile and Fish Medicine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria.
Exp Parasitol. 2010 Feb;124(2):202-8. doi: 10.1016/j.exppara.2009.09.014. Epub 2009 Sep 18.
A rapid and simple procedure was established to obtain clonal axenic cultures of Tetratrichomonas gallinarum and Trichomonas gallinae and to optimize their in vitro growth conditions. Medium 199 was used for axenization of two genetically different clones of T. gallinarum and T. gallinae. Six different media were used to optimize the growth behaviour of axenically grown parasites: Medium 199, TYM, TYI-S-33, Hollander fluid (HF), Trichomonas vaginalis (TV) and modified TV media. The highest cell yields for both axenic clones of T. gallinarum were obtained in modified TV medium without antibiotics. The maximum numbers of trophozoites of T. gallinae were obtained in an optimized HF medium. This study demonstrated that axenic cultures for T. gallinarum and T. gallinae could be obtained avoiding the migration technique through a V-tube. Following axenization and optimization, both clones of T. gallinarum and T. gallinae could be propagated both aerobically and anaerobically.
建立了一种快速而简单的方法，可获得鸡四鞭毛虫和鸡滴虫的克隆无菌培养物，并优化其体外生长条件。使用 199 号培养基对两种遗传上不同的鸡四鞭毛虫和鸡滴虫克隆进行无菌化处理。使用六种不同的培养基来优化无菌培养寄生虫的生长行为：199 号培养基、TYM、TYI-S-33、霍兰德培养液（HF）、阴道毛滴虫（TV）和改良 TV 培养基。两种鸡四鞭毛虫无菌克隆的最高细胞产量均在不含抗生素的改良 TV 培养基中获得。鸡滴虫的最大滋养体数量在优化的 HF 培养基中获得。本研究表明，可通过避免通过 V 型管进行迁移技术来获得鸡四鞭毛虫和鸡滴虫的无菌培养物。无菌化和优化后，两种鸡四鞭毛虫和鸡滴虫克隆均可在有氧和无氧条件下繁殖。
