一种用于同时检测和区分 和 的新型双重实时 PCR。
A new duplex real-time PCR for simultaneous detection and differentiation of and .
机构信息
Department of Poultry and Rabbit Diseases, Institute for Food Safety and Hygiene, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.
出版信息
J Vet Diagn Invest. 2022 Jul;34(4):631-637. doi: 10.1177/10406387221098069. Epub 2022 May 20.
Abstract
and are pathogenic avian parasites that infect a wide range of bird species. The pathologic potential of is controversial, whereas causes disease in many avian species. Infections are often asymptomatic in doves and pigeons; thus, columbids are presumed to represent the natural hosts for trichomonads. The detection of and is based on direct microscopic observation or a conventional PCR assay. Microscopy is not very sensitive, and identification of the trichomonads at the genus or species level is not possible. Conventional PCR assays have been developed primarily for phylogenetic studies, which detect a wide range of spp. but do not allow their differentiation. We developed a duplex real-time PCR (rtPCR) assay for the simultaneous detection and differentiation of and . We found that the rtPCR assay detected 10 plasmid DNA copies of and as few as 10 plasmid DNA copies of
摘要
和 是致病性的禽类寄生虫，可感染多种鸟类。 的病理潜能存在争议，而 可导致多种禽类患病。在鸽子和斑鸠中，感染通常无症状；因此，鸽形目被认为是滴虫的自然宿主。 和 的检测基于直接显微镜观察或常规 PCR 检测。显微镜观察的灵敏度不高，无法确定滴虫的属或种水平。常规 PCR 检测主要用于系统发育研究，可检测广泛的 属，但不能区分它们。我们开发了一种用于 和 的同时检测和区分的双重实时 PCR（rtPCR）检测法。我们发现，该 rtPCR 检测法可检测到 10 个质粒 DNA 拷贝的 和低至 10 个质粒 DNA 拷贝的
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