Pirmez C, Cooper C, Paes-Oliveira M, Schubach A, Torigian V K, Modlin R L
Evandro Chagas Hospital, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil.
J Immunol. 1990 Nov 1;145(9):3100-4.
American cutaneous leishmaniasis is a disease of skin and mucous membranes in which T lymphocytes reactive to Leishmania (Viannia) braziliensis are thought to contribute to protective immunity. To characterize the nature of the T cell inflammatory infiltrate in American cutaneous leishmaniasis lesions, immunohistochemistry with mAb that define T cell subpopulations and in situ hybridization to detect mRNA coding for IFN-gamma were performed. In both localized cutaneous (LCL) and mucocutaneous (MCL) lesions, we observed a predominance of T memory (CD4+CD45RO+) as compared to T naive cells (CD+CD45RA+). The percentages of cells containing IFN-gamma mRNA were equivalent in both LCL and MCL lesions. T cells were extracted from LCL and MCL lesions and analysis indicated that T cells from both lesions had been stimulated by L. (V.) braziliensis in vivo and gave equivalent proliferative responses in vitro. The present data suggest that T memory cells, which are likely to elaborate IFN-gamma, are components of DTH response to L. (V.) braziliensis and participate in the pathogenesis of both LCL and MCL lesions.
美洲皮肤利什曼病是一种皮肤和黏膜疾病，对巴西利什曼原虫（维氏亚属）产生反应的T淋巴细胞被认为有助于产生保护性免疫。为了明确美洲皮肤利什曼病皮损中T细胞炎性浸润的性质，我们使用了可界定T细胞亚群的单克隆抗体进行免疫组化，并通过原位杂交检测编码γ干扰素的mRNA。在局限性皮肤（LCL）和黏膜皮肤（MCL）皮损中，与初始T细胞（CD4+CD45RA+）相比，我们观察到T记忆细胞（CD4+CD45RO+）占优势。LCL和MCL皮损中含有γ干扰素mRNA的细胞百分比相当。从LCL和MCL皮损中提取T细胞，分析表明，来自这两种皮损的T细胞在体内均受到巴西利什曼原虫（维氏亚属）的刺激，并且在体外产生相当的增殖反应。目前的数据表明，可能产生γ干扰素的T记忆细胞是对巴西利什曼原虫（维氏亚属）迟发型超敏反应的组成部分，并参与LCL和MCL皮损的发病机制。
