Richardson J H, Edwards A J, Cruickshank J K, Rudge P, Dalgleish A G
Department of Transplantation Biology, MRC Clinical Research Centre, Harrow, Middlesex, United Kingdom.
J Virol. 1990 Nov;64(11):5682-7. doi: 10.1128/JVI.64.11.5682-5687.1990.
To establish the phenotype of human T-cell leukemia virus type 1 (HTLV-1)-infected cells in peripheral blood, the polymerase chain reaction was used to detect and quantitate viral DNA in subpopulations of leukocytes obtained from patients with tropical spastic paraparesis and asymptomatic carriers. HTLV-1 could not be detected in peripheral blood mononuclear cells thoroughly depleted of T lymphocytes (E- CD3-), nor could it be detected in highly enriched populations of B lymphocytes (E- CD19+), monocytes (E- CD14+), or natural killer cells (E- CD16+). T lymphocytes were strongly positive for HTLV-1, and fractionation of this population revealed that 90 to 99% of the HTLV-1 DNA segregated with the CD4+ CD8- and CD45RO+ subsets. No difference between the cell type distribution of HTLV-1 in the asymptomatic carrier and the subjects with tropical spastic paraparesis was evident. Southern hybridization of genomic DNA prepared from the peripheral blood of HTLV-1 carriers indicated that up to 10% of circulating leukocytes may carry the HTLV-1 provirus.
为确定外周血中人类嗜T淋巴细胞病毒1型（HTLV-1）感染细胞的表型，采用聚合酶链反应检测并定量从热带痉挛性截瘫患者和无症状携带者获取的白细胞亚群中的病毒DNA。在彻底清除T淋巴细胞的外周血单核细胞（E-CD3-）中未检测到HTLV-1，在高度富集的B淋巴细胞（E-CD19+）、单核细胞（E-CD14+）或自然杀伤细胞（E-CD16+）群体中也未检测到。T淋巴细胞对HTLV-1呈强阳性，对该群体进行分级分离显示，90%至99%的HTLV-1 DNA与CD4+CD8-和CD45RO+亚群相关。无症状携带者和热带痉挛性截瘫患者中HTLV-1的细胞类型分布无明显差异。对HTLV-1携带者外周血制备的基因组DNA进行Southern杂交表明，高达10%的循环白细胞可能携带HTLV-1前病毒。
