Hoxie J A, Matthews D M, Cines D B
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7591-5. doi: 10.1073/pnas.81.23.7591.
The effects of the human T-cell leukemia virus type I (HTLV-I) on cultured human endothelial cells were evaluated. Coculture of endothelial monolayers with either irradiated HTLV-producing lymphocytes or cell-free virus resulted in the production of multinucleated syncytia. The development of syncytia was inhibited by sera from patients with adult T-cell leukemia/lymphoma (ATLL). HTLV antigens were present on endothelial syncytia passaged in culture for greater than 3 months as detected by an anti-p19 monoclonal antibody, which detects a core protein of HTLV-I, and by ATLL sera. Moreover, these HTLV-infected endothelial cells were then able to infect and transform normal cord blood T lymphocytes with HTLV. These studies demonstrate that human endothelial cells are susceptible to productive HTLV-I infection in vitro and may have relevance for the spectrum of human disease associated with this family of retroviruses.
对人类I型T细胞白血病病毒（HTLV-I）对培养的人内皮细胞的影响进行了评估。将内皮细胞单层与经辐照的产生HTLV的淋巴细胞或无细胞病毒共培养，导致多核巨细胞的产生。来自成人T细胞白血病/淋巴瘤（ATLL）患者的血清可抑制巨细胞的形成。通过检测HTLV-I核心蛋白的抗p19单克隆抗体以及ATLL血清检测发现，在培养超过3个月的内皮巨细胞上存在HTLV抗原。此外，这些被HTLV感染的内皮细胞随后能够用HTLV感染并转化正常脐血T淋巴细胞。这些研究表明，人内皮细胞在体外易受HTLV-I的有效感染，并且可能与这一逆转录病毒家族相关的人类疾病谱有关。
