Xi Yajun, Ge Yaxin, Wang Zeng-Yu
Northwest A and F University, Yangling, Shaanxi, China.
Methods Mol Biol. 2009;581:53-9. doi: 10.1007/978-1-60761-214-8_4.
Switchgrass (Panicum virgatum L.) is a highly productive warm-season C4 species that is being developed into a dedicated biofuel crop. This chapter describes a protocol that allows the generation of transgenic switchgrass plants by Agrobacterium tumefaciens-mediated transformation. Embryogenic calluses induced from caryopses or inflorescences were used as explants for inoculation with A. tumefaciens strain EHA105. Hygromycin phosphotransferase gene (hph) was used as the selectable marker and hygromycin was used as the selection agent. Calluses resistant to hygromycin were obtained after 5-6 weeks of selection. Soil-grown switchgrass plants were regenerated about 6 months after callus induction and Agrobacterium-mediated transformation.
柳枝稷（Panicum virgatum L.）是一种高产的暖季型C4植物，正被开发成为一种专用生物燃料作物。本章描述了一种通过根癌农杆菌介导的转化来培育转基因柳枝稷植株的方法。由颖果或花序诱导产生的胚性愈伤组织用作外植体，与根癌农杆菌菌株EHA105进行接种。潮霉素磷酸转移酶基因（hph）用作选择标记，潮霉素用作选择剂。经过5 - 6周的筛选后获得了对潮霉素具有抗性的愈伤组织。在愈伤组织诱导和农杆菌介导的转化后约6个月，再生出了在土壤中生长的柳枝稷植株。
