University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstr. 30, 48149 Münster, Germany.
Anal Bioanal Chem. 2009 Nov;395(6):1929-35. doi: 10.1007/s00216-009-3105-1.
To investigate the effects of mercury species intoxication and to test the efficiency of different commonly applied antidotes, human whole blood and plasma surrogate samples were spiked with inorganic mercury (Hg2+) and methylmercury (MeHg+, CH3Hg+) prior to treatment with the antidotes 2,3-dimercaptopropan-1-ol (British Anti Lewisite), 2,3-dimercaptosuccinic acid (DMSA), and N-acetylcysteine (NAC). For mercury speciation analysis in these samples, liquid chromatography was coupled to either inductively coupled plasma mass spectrometry (ICP-MS) or electrospray ionisation time-of-flight mass spectrometry (ESI-TOF-MS). Adduct formation between mercury species and physiological thiols (cysteine and glutathione) was observed as well as the release of glutathione under treatment with the antidotes DMSA and NAC.
为了研究汞形态中毒的影响，并测试不同常用解毒剂的效率，在用人全血和血浆替代物样本进行处理之前，向其中加入无机汞（Hg2+）和甲基汞（MeHg+，CH3Hg+），然后用 2,3-二巯基丙醇（British Anti Lewisite）、2,3-二巯基丁二酸（DMSA）和 N-乙酰半胱氨酸（NAC）进行解毒。对于这些样品中的汞形态分析，采用液相色谱法与电感耦合等离子体质谱法（ICP-MS）或电喷雾离子化飞行时间质谱法（ESI-TOF-MS）相结合。观察到汞形态与生理硫醇（半胱氨酸和谷胱甘肽）之间形成加合物，以及解毒剂 DMSA 和 NAC 处理下谷胱甘肽的释放。
