Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, USA.
J Biomed Mater Res A. 2010 Sep 15;94(4):1195-204. doi: 10.1002/jbm.a.32802.
A family of methacrylic terpolymer biomaterials was electrospun into three-dimensional fibrous scaffolds. The glass transition temperature of the polymer correlates with the morphology of the resulting scaffold. Glassy materials produce scaffolds with discrete fibers and a high percent void space (84%) while the rubbery materials produced scaffolds with fused fibers and a much lower percent void space (18%). By electrospinning onto a rotating mandrel, aligned fiber scaffolds were fabricated, and it was shown that controlling the rotation speed of the collector allowed for control over the degree of fiber alignment. The electrospinning was shown to not degrade the number average molecular weight of the polymer chains. Human umbilical vein endothelial cells (HUVECs) were seeded onto the electrospun scaffolds under static conditions and it was found that the morphology of the scaffold controlled the cellular proliferation, the metabolic activity, and the morphology of adherent cells. In particular, HUVECs seeded onto low void space scaffolds exhibited enhanced cellular spreading, enzymatic activity, and proliferation. HUVECs seeded onto aligned fiber scaffolds did not demonstrate increased proliferation; however, the cells did organize themselves in the direction of fiber alignment resulting in cells with elongated morphology.
将甲基丙烯酸酯三元共聚物生物材料家族电纺成三维纤维支架。聚合物的玻璃化转变温度与所得支架的形态相关。玻璃态材料产生具有离散纤维和高百分比空隙（84%）的支架，而橡胶态材料则产生具有融合纤维和低得多的百分比空隙（18%）的支架。通过在旋转心轴上进行静电纺丝，可以制备出取向纤维支架，并且表明控制收集器的旋转速度可以控制纤维取向的程度。静电纺丝未降低聚合物链的数均分子量。将人脐静脉内皮细胞（HUVEC）在静态条件下接种到静电纺丝支架上，结果发现支架的形态控制着细胞的增殖、代谢活性和贴壁细胞的形态。特别是，接种到低空隙支架上的 HUVEC 表现出增强的细胞扩展、酶活性和增殖。接种到取向纤维支架上的 HUVEC 并未表现出增殖增加；然而，细胞确实在纤维取向的方向上自我组织，导致细胞具有拉长的形态。
