可变剪接调控机制：来自分子和基因组学方法的见解
Mechanisms of alternative splicing regulation: insights from molecular and genomics approaches.
作者信息
Chen Mo, Manley James L
机构信息
Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
出版信息
Nat Rev Mol Cell Biol. 2009 Nov;10(11):741-54. doi: 10.1038/nrm2777. Epub 2009 Sep 23.
Abstract
Alternative splicing of mRNA precursors provides an important means of genetic control and is a crucial step in the expression of most genes. Alternative splicing markedly affects human development, and its misregulation underlies many human diseases. Although the mechanisms of alternative splicing have been studied extensively, until the past few years we had not begun to realize fully the diversity and complexity of alternative splicing regulation by an intricate protein-RNA network. Great progress has been made by studying individual transcripts and through genome-wide approaches, which together provide a better picture of the mechanistic regulation of alternative pre-mRNA splicing.
摘要
mRNA前体的可变剪接提供了一种重要的基因调控方式，并且是大多数基因表达过程中的关键步骤。可变剪接显著影响人类发育，其调控异常是许多人类疾病的根源。尽管可变剪接的机制已得到广泛研究，但直到过去几年，我们才开始充分认识到由复杂的蛋白质-RNA网络调控可变剪接的多样性和复杂性。通过研究单个转录本以及全基因组方法已经取得了巨大进展，这些研究共同为前体mRNA可变剪接的机制调控提供了更清晰的图景。
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本文引用的文献
1
A regulator of Dscam mutually exclusive splicing fidelity.
Nat Struct Mol Biol. 2007 Dec;14(12):1134-40. doi: 10.1038/nsmb1339.
2
DNA damage regulates alternative splicing through inhibition of RNA polymerase II elongation.
Cell. 2009 May 15;137(4):708-20. doi: 10.1016/j.cell.2009.03.010.
3
SRp38 regulates alternative splicing and is required for Ca(2+) handling in the embryonic heart.
Dev Cell. 2009 Apr;16(4):528-38. doi: 10.1016/j.devcel.2009.02.009.
4
ESRP1 and ESRP2 are epithelial cell-type-specific regulators of FGFR2 splicing.
Mol Cell. 2009 Mar 13;33(5):591-601. doi: 10.1016/j.molcel.2009.01.025.
5
Genome-wide analysis of alternative pre-mRNA splicing and RNA-binding specificities of the Drosophila hnRNP A/B family members.
Mol Cell. 2009 Feb 27;33(4):438-49. doi: 10.1016/j.molcel.2009.01.022.
6
Differential 3' splice site recognition of SMN1 and SMN2 transcripts by U2AF and U2 snRNP.
RNA. 2009 Apr;15(4):515-23. doi: 10.1261/rna.1273209. Epub 2009 Feb 25.
7
The spliceosome: design principles of a dynamic RNP machine.
Cell. 2009 Feb 20;136(4):701-18. doi: 10.1016/j.cell.2009.02.009.
8
Riboswitch RNAs: using RNA to sense cellular metabolism.
Genes Dev. 2008 Dec 15;22(24):3383-90. doi: 10.1101/gad.1747308.
9
An RNA code for the FOX2 splicing regulator revealed by mapping RNA-protein interactions in stem cells.
Nat Struct Mol Biol. 2009 Feb;16(2):130-7. doi: 10.1038/nsmb.1545. Epub 2009 Jan 11.
10
Splicing factor SFRS1 recognizes a functionally diverse landscape of RNA transcripts.
Genome Res. 2009 Mar;19(3):381-94. doi: 10.1101/gr.082503.108. Epub 2008 Dec 30.
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