Budowle B, Baechtel F S, Giusti A M, Monson K L
Forensic Science Research and Training Center, Laboratory Division, FBI Academy, Quantico, VA 22135.
Clin Biochem. 1990 Aug;23(4):287-93. doi: 10.1016/0009-9120(90)80055-n.
The detection of alleles of variable number of tandem repeats (VNTR) loci by restriction fragment length polymorphism analysis has become an important aspect of genetic characterization for identity testing. Some VNTR loci are so polymorphic that the analysis of three to five genetic markers could potentially provide unique identity. However, the more informative a genetic marker is (i.e., high degree of polymorphisms), the better it is as an exculpatory tool. This approach currently provides the best avenue for excluding a falsely associated individual with a particular sample. When an analysis fails to exclude an individual as the source of the questioned material, a value (frequency of occurrence) should be placed on the VNTR profiles to assess weight to the inclusion in identity testing. Arbitrarily defined fixed bins were designed to accommodate quasi-continuous data and to provide a result that would not place an underestimation of the frequency of occurrence of a set of alleles attributed to an individual.
通过限制性片段长度多态性分析来检测可变数目串联重复序列（VNTR）位点的等位基因，已成为身份鉴定中基因特征分析的一个重要方面。一些VNTR位点具有高度多态性，以至于分析三到五个遗传标记就有可能提供独特的身份信息。然而，一个遗传标记的信息量越大（即多态性程度越高），它作为免责工具就越好。这种方法目前为排除与特定样本错误关联的个体提供了最佳途径。当分析未能排除某一个体作为可疑材料来源时，应给VNTR图谱赋予一个值（出现频率），以评估在身份鉴定中该包含结论的权重。任意定义的固定区间旨在容纳准连续数据，并提供一个不会低估归属于某一个体的一组等位基因出现频率的结果。
