Reproductive Medicine Unit, GynePro Medical Centers, Bologna, Italy.
Reprod Biomed Online. 2009 Sep;19(3):374-9. doi: 10.1016/s1472-6483(10)60171-x.
This multi-centre study evaluated systematically the influence of the duration of cryostorage on the outcome of thawing cycles when using slow-frozen oocytes. The thawing cycles were retrospectively divided into three main groups based on cryostorage duration: group A, 1-3 months; group B, 4-6 months; and group C, 7-48 months. Group C was subsequently divided into three subgroups: group C1, 7-9 months; group C2, 10-12 months; and group C3, 13-48 months. Main outcome measures observed were oocyte survival after thawing, fertilization, cleavage; embryo quality and development, implantation, and birth. No significant differences in main outcome measures were observed between all the groups studied. In conclusion, human oocytes can be safely cryostored for several years. This finding could encourage the wider use of oocyte cryopreservation as a clinical procedure in assisted reproduction.
这项多中心研究系统评估了慢速冷冻卵子解冻周期中冷冻保存时间对结果的影响。根据冷冻保存时间，将解冻周期分为三组：A 组，1-3 个月；B 组，4-6 个月；C 组，7-48 个月。C 组随后分为三个亚组：C1 组，7-9 个月；C2 组，10-12 个月；C3 组，13-48 个月。主要观察指标为解冻后卵子存活率、受精、卵裂；胚胎质量和发育、着床和分娩。研究中所有组间主要观察指标均无显著差异。结论：人类卵子可安全冷冻保存数年。这一发现可能会鼓励更广泛地将卵子冷冻保存作为辅助生殖中的临床程序。
