Reproductive Medicine Center, Affiliated Shenzhen City Maternity and Child Healthcare Hospital of Southern Medical University, Shenzhen, China.
Department of Reproductive Health and Infertility, Guangdong Women and Children Hospital, Guangzhou, Guangdong, China.
J Obstet Gynaecol Res. 2022 Dec;48(12):3152-3159. doi: 10.1111/jog.15412. Epub 2022 Sep 13.
To investigate the effectiveness of oocyte thawing cycles in the clinical application of assisted reproductive technology (ART).
The clinical data of 78 cases who underwent oocyte thawing cycles in our center were retrospectively analyzed. All patients in this study received oocyte cryopreservation for the husband reason. According to patient age at egg freezing, patients were divided into three observation groups (Group A, <30 years old; Group B, 30-34 years old; Group C, ≥35 years old), and the control groups were selected by propensity score matching with fresh cycles. The clinical outcomes of each group were compared, and the clinical efficacy of oocyte thawing cycles was analyzed.
Clinical pregnancy outcomes of oocyte thawing cycles were not significantly different from that of fresh oocytes, but vitrification affected the number of two pronuclei zygotes developing to cleavage stage and the number of high-quality embryos, and the normal fertilization rate after thawing. The cycle cumulative live birth rate in Group C was significantly lower than those in Groups A and B. The live birth rates per egg of Groups A, B, C were 5.03%, 5.61%, and 3.57%, respectively, and the numbers of eggs per live birth were 13.72, 14.43, and 21.0, respectively.
The overall clinical outcomes of oocyte vitrification were similar to that of fresh oocytes, but the cleavage rate and embryo quality of frozen oocytes were slightly reduced. Freezing of oocytes in women over 35 years of age affects the clinical efficacy of ART.
探讨卵母细胞解冻周期在辅助生殖技术（ART）临床应用中的效果。
回顾性分析本中心 78 例行卵母细胞解冻周期的临床资料。本研究所有患者均因男方原因行卵母细胞冷冻保存。根据患者冻卵时的年龄，将患者分为三组观察（A 组，<30 岁；B 组，30-34 岁；C 组，≥35 岁），并通过倾向性评分匹配选择新鲜周期的对照组。比较各组的临床结局，分析卵母细胞解冻周期的临床疗效。
卵母细胞解冻周期的临床妊娠结局与新鲜卵母细胞无显著差异，但玻璃化冷冻对形成二倍体合子的卵裂期胚胎数量和优质胚胎数量以及解冻后的正常受精率有影响。C 组的周期累积活产率明显低于 A 组和 B 组。A、B、C 组的每卵活产率分别为 5.03%、5.61%和 3.57%，每活产卵数分别为 13.72、14.43 和 21.0。
卵母细胞玻璃化冷冻的总体临床结局与新鲜卵母细胞相似，但冷冻卵母细胞的卵裂率和胚胎质量略有下降。35 岁以上女性的卵母细胞冷冻会影响 ART 的临床疗效。
