Institute of Biomedicine/Medical Biochemistry and Developmental Biology, Genome-Scale Biology Research Program, University of Helsinki, Helsinki, Finland.
Genes Chromosomes Cancer. 2010 Jan;49(1):28-39. doi: 10.1002/gcc.20715.
To elucidate gene expression signatures associated with gastric carcinogenesis, we performed a genome-wide expression analysis of 46 Finnish and 20 Japanese gastric tissues. Comparative analysis between Finnish and Japanese datasets identified 58 common genes that were differentially expressed between cancerous and non-neoplastic gastric tissues. Twenty-six of these genes were up-regulated in cancer and 32 down-regulated. Of these genes, 64% were also differentially expressed in another unrelated publicly available dataset. The expression levels of four of the up-regulated genes, CXCL1, SPARC, SPP1 and SULF, were further analyzed in 82 gastric tissues using quantitative real-time RT-PCR. This analysis validated the results from the microarray analysis as the expression of these four genes was significantly higher in the cancerous tissue compared with the normal tissue (fold change 3.4-8.9). Over-expression of CXCL1 also positively correlated with improved survival. To conclude, irrespective of the microarray platform or patient population, a common gastric cancer gene expression signature of 58 genes, including CXCL1, SPARC, SPP1, and SULF, was identified. These genes represent potential biomarkers for gastric cancer.
为了阐明与胃癌发生相关的基因表达特征，我们对 46 份芬兰和 20 份日本胃组织进行了全基因组表达分析。芬兰和日本数据集之间的比较分析确定了 58 个在癌组织和非肿瘤性胃组织之间差异表达的共同基因。这些基因中有 26 个在癌症中上调，32 个下调。其中，这些基因中有 64%在另一个不相关的公开可用数据集也存在差异表达。使用定量实时 RT-PCR 在 82 个胃组织中进一步分析了上调的四个基因（CXCL1、SPARC、SPP1 和 SULF）的表达水平。该分析验证了微阵列分析的结果，因为这四个基因在癌组织中的表达明显高于正常组织（倍数变化 3.4-8.9）。CXCL1 的过度表达也与改善的生存呈正相关。总之，无论使用微阵列平台还是患者人群，都鉴定出了包括 CXCL1、SPARC、SPP1 和 SULF 在内的 58 个共同的胃癌基因表达特征。这些基因代表了胃癌的潜在生物标志物。
