Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, South Korea.
Immunol Invest. 2011;40(2):160-71. doi: 10.3109/08820139.2010.525570. Epub 2010 Nov 10.
To investigate whether netrin-1 is involved in autoimmune injury of the central nervous system, the expression of netrin-1 protein was analyzed in the spinal cord of Lewis rats with experimental autoimmune encephalomyelitis (EAE). Western blot analysis revealed significantly increased content of netrin-1 in the spinal cords of rats at the peak stage of EAE, as compared with the levels in normal control animals (p < 0.01). Immunohistochemistry detected the netrin-1 protein in neurons, oligodendrocytes, astrocytes and vascular endothelial cells in the spinal cords of normal controls. In EAE-affected spinal cords, netrin-1 immunoreactivity was detected in infiltrating inflammatory cells at the peak stage as well as in neurons, oligodendrocytes and astrocytes. These results suggest that netrin-1 is transiently increased in rat EAE lesions, where it contributes to the modulation of rat acute EAE.
为了研究 netrin-1 是否参与中枢神经系统的自身免疫损伤，我们分析了实验性自身免疫性脑脊髓炎（EAE）大鼠脊髓中 netrin-1 蛋白的表达。Western blot 分析显示，EAE 高峰期大鼠脊髓中 netrin-1 的含量明显高于正常对照组（p<0.01）。免疫组化检测到正常对照组大鼠脊髓中的神经元、少突胶质细胞、星形胶质细胞和血管内皮细胞中存在 netrin-1 蛋白。在 EAE 受累的脊髓中，在高峰期浸润的炎症细胞以及神经元、少突胶质细胞和星形胶质细胞中均可检测到 netrin-1 免疫反应性。这些结果表明，netrin-1 在大鼠 EAE 病变中短暂增加，有助于调节大鼠急性 EAE。
