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利用疼痛异质性和 RNA 转录组鉴定基于血液的疼痛生物标志物：在分级慢性缩窄性损伤模型中一种新的相关性研究设计和生物信息学方法。

Harnessing pain heterogeneity and RNA transcriptome to identify blood-based pain biomarkers: a novel correlational study design and bioinformatics approach in a graded chronic constriction injury model.

机构信息

Discipline of Pharmacology, School of Medical Sciences, University of Adelaide, Adelaide, Australia.

出版信息

J Neurochem. 2012 Sep;122(5):976-94. doi: 10.1111/j.1471-4159.2012.07833.x. Epub 2012 Jul 9.

DOI:10.1111/j.1471-4159.2012.07833.x
PMID:22697386
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5518237/
Abstract

A quantitative, peripherally accessible biomarker for neuropathic pain has great potential to improve clinical outcomes. Based on the premise that peripheral and central immunity contribute to neuropathic pain mechanisms, we hypothesized that biomarkers could be identified from the whole blood of adult male rats, by integrating graded chronic constriction injury (CCI), ipsilateral lumbar dorsal quadrant (iLDQ) and whole blood transcriptomes, and pathway analysis with pain behavior. Correlational bioinformatics identified a range of putative biomarker genes for allodynia intensity, many encoding for proteins with a recognized role in immune/nociceptive mechanisms. A selection of these genes was validated in a separate replication study. Pathway analysis of the iLDQ transcriptome identified Fcγ and Fcε signaling pathways, among others. This study is the first to employ the whole blood transcriptome to identify pain biomarker panels. The novel correlational bioinformatics, developed here, selected such putative biomarkers based on a correlation with pain behavior and formation of signaling pathways with iLDQ genes. Future studies may demonstrate the predictive ability of these biomarker genes across other models and additional variables.

摘要

一种定量的、可用于外周的神经病理性疼痛生物标志物具有改善临床结果的巨大潜力。基于外周和中枢免疫有助于神经病理性疼痛机制的前提，我们假设通过整合分级慢性缩窄性损伤（CCI）、同侧腰椎背象限（iLDQ）和全血转录组以及疼痛行为的通路分析，从成年雄性大鼠的全血中可以鉴定出生物标志物。相关性生物信息学确定了一系列用于评估痛觉过敏强度的潜在生物标志物基因，其中许多基因编码在免疫/伤害感受机制中具有公认作用的蛋白质。这些基因中的一部分在单独的复制研究中得到了验证。iLDQ 转录组的通路分析确定了 Fcγ 和 Fcε 信号通路等。本研究首次采用全血转录组来鉴定疼痛生物标志物谱。这里开发的新相关性生物信息学方法是根据与疼痛行为的相关性以及与 iLDQ 基因形成的信号通路来选择这些潜在生物标志物的。未来的研究可能会证明这些生物标志物基因在其他模型和其他变量中的预测能力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0afd/5518237/8e5500f6a91d/nihms877859f7.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0afd/5518237/b9b9032259d9/nihms877859f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0afd/5518237/a04b025f39a2/nihms877859f2.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0afd/5518237/8e5500f6a91d/nihms877859f7.jpg

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1
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2
Pain sensitivity and vasopressin analgesia are mediated by a gene-sex-environment interaction.疼痛敏感性和血管加压素镇痛是由基因-性别-环境相互作用介导的。
Nat Neurosci. 2011 Oct 23;14(12):1569-73. doi: 10.1038/nn.2941.
3
Spinal P2X(7) receptor mediates microglia activation-induced neuropathic pain in the sciatic nerve injury rat model.脊髓 P2X（7）受体介导坐骨神经损伤大鼠模型中小胶质细胞激活诱导的神经性疼痛。
自身反应性IgG水平和Fc受体γ亚基上调在神经缩窄或挤压损伤后引发机械性异常性疼痛。
bioRxiv. 2025 Mar 24:2025.03.22.644748. doi: 10.1101/2025.03.22.644748.
4
B cells drive neuropathic pain-related behaviors in mice through IgG-Fc gamma receptor signaling.B 细胞通过 IgG-Fcγ 受体信号传导驱动小鼠的神经病理性疼痛相关行为。
Sci Transl Med. 2024 Sep 25;16(766):eadj1277. doi: 10.1126/scitranslmed.adj1277.
5
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6
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Front Neurosci. 2022 May 19;16:887042. doi: 10.3389/fnins.2022.887042. eCollection 2022.
7
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