Underwood John R, Chivers Mark, Dang Thi Thuong, Licciardi Paul V
Department of Pathology, University of Melbourne, Parkville, Victoria 3010, Australia.
Vaccine. 2009 Nov 5;27(47):6634-41. doi: 10.1016/j.vaccine.2009.03.051. Epub 2009 Apr 8.
The immunomodulatory properties of botanical medicinals are well-documented. In this study, the capacity for the traditional Chinese herbal medicine, Rehmannia Six Formula (R6F), to stimulate anti-tetanus toxoid (TT) immunity following oral administration to mice was examined. A significant rise in serum anti-TT antibody levels were observed in R6F-treated mice immunized with a minimum immunogenic dose of 10 microg TT suggesting an oral adjuvant effect. No such enhancement was found for unimmunized mice treated with R6F. This anti-TT response was preferentially IgG and antigen-specific in relation to antibody reactivity to a panel of unrelated antigens. The R6F used was safe with no adverse effects on mouse weight or survival, providing evidence for the use of R6F as an oral adjuvant.
植物药的免疫调节特性已有充分记载。在本研究中，检测了口服给予小鼠传统中药六味地黄丸（R6F）后刺激抗破伤风类毒素（TT）免疫的能力。在用最低免疫原剂量10微克TT免疫的R6F处理小鼠中，观察到血清抗TT抗体水平显著升高，提示口服佐剂效应。在用R6F处理的未免疫小鼠中未发现这种增强作用。这种抗TT反应相对于对一组无关抗原的抗体反应性而言，优先为IgG且具有抗原特异性。所用的R6F是安全的，对小鼠体重或存活没有不良影响，为将R6F用作口服佐剂提供了证据。
