Department of Environmental and Natural Resources Management, University of Ioannina, G. Seferi 2, 30100 Agrinio, Greece.
Bioresour Technol. 2010 Feb;101(4):1385-8. doi: 10.1016/j.biortech.2009.09.028. Epub 2009 Sep 24.
A semi-solid fermentation process for the production of biodiesel from sweet sorghum is introduced. The microorganism used is the oleaginous fungus Mortierella isabellina, which is able to transform efficiently sugar to storage lipid. Kinetic experiments were performed at various water content percentages. The fungus consumed simultaneously sugars and nitrogen contained in sorghum and after nitrogen depletion the biomass growth was completed and oil accumulation began. Water content of 92% presented the highest oil efficiency of 11 g/100 g dry weight of substrate. The semi-solid process is shown to have certain advantages compared to liquid cultures or solid-state fermentation and gives oil of high quality.
从甜高粱生产生物柴油的半固态发酵工艺。所用的微生物是产油真菌玫烟色拟青霉，它能有效地将糖转化为储存脂质。在不同的含水量下进行了动力学实验。真菌同时消耗高粱中的糖和氮，氮耗尽后，生物量生长完成，油脂开始积累。含水量为 92%时，底物的干重产油效率最高，为 11g/100g。与液体培养或固态发酵相比，半固态工艺具有一定的优势，可得到高质量的油脂。
