National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
Bioresour Technol. 2011 Dec;102(24):11262-5. doi: 10.1016/j.biortech.2011.09.103. Epub 2011 Oct 1.
A solid state fermentation (SSF) of sweet sorghum stalk to ethanol was conducted in 250-mL flask using thermotolerant Issatchenkia orientalis IPE 100, and the optimal operation parameters were determined as 42°C fermentation temperature, 75% (w/w) water content, 2mm particle size and 3% (w/w) inoculation rate in 250-mL conical flask. When the SSF was scaled up from the flask to a 10-L bioreactor, temperature gradient in the substrate bed was observed due to heat accumulation in the bioreactor. The temperature gradient was dependent on both substrate depth and operation temperature. Due to high thermotolerance of the strain IPE 100, a deep-bed SSF of sweet sorghum stalk was developed in the bioreactor. The highest ethanol yield of 0.25 g-ethanol/g-dry stalk was obtained at 37°C with 15-20 cm substrate depth in the bioreactor. These results provided a great potential for large-scale deep-bed SSF in practice.
采用耐热东方伊萨酵母 IPE 100 在 250-mL 锥形瓶中进行甜高粱秸秆固态发酵（SSF）生产乙醇，确定了最佳的操作参数为：发酵温度 42°C、水含量 75%（w/w）、颗粒大小 2mm 和接种率 3%（w/w）。当 SSF 从小锥形瓶放大到 10-L 生物反应器时，由于生物反应器中热量积累，观察到基质床中的温度梯度。温度梯度取决于基质深度和操作温度。由于菌株 IPE 100 的高耐热性，在生物反应器中开发了深层 SSF 生产甜高粱秸秆。在生物反应器中，底物深度为 15-20cm 时，在 37°C 下可获得最高的乙醇产量为 0.25g-乙醇/g-干秸秆。这些结果为大规模深层 SSF 在实际中的应用提供了巨大潜力。
