Dipartimento di Fisica, ULTRAS-CNR-INFM and IFN-CNR, Politecnico di Milano, I-20133 Milan, Italy.
Talanta. 2009 Nov 15;80(1):286-93. doi: 10.1016/j.talanta.2009.06.063. Epub 2009 Jul 4.
The analysis of various varnishes from different origins, which are commonly found on historical musical instruments was carried out for the first time with both fluorescence excitation emission spectroscopy and laser-induced time-resolved fluorescence spectroscopy. Samples studied include varnishes prepared using shellac, and selected diterpenoid and triterpenoid resins from plants, and mixtures of these materials. Fluorescence excitation emission spectra have been collected from films of naturally aged varnishes. In parallel, time-resolved fluorescence spectroscopy of varnishes provides means for discriminating between short- (less than 2.0 ns) and long-lived (greater than 7.5 ns) fluorescence emissions in each of these complex materials. Results suggest that complementary use of the two non destructive techniques allows a better understanding of the main fluorophores responsible for the emission in shellac, and further provides means for distinguishing the main classes of other varnishes based on differences in fluorescence lifetime behaviour. Spectrofluorimetric data and time resolved spectra presented here may form the basis for the interpretation of results from future in situ fluorescence examination and time resolved fluorescence imaging of varnished musical instruments.
首次采用荧光激发发射光谱和激光诱导时间分辨荧光光谱法对来自不同来源的各种常见于历史乐器上的清漆进行了分析。研究的样品包括使用紫胶、从植物中选择的二萜和三萜树脂以及这些材料的混合物制备的清漆。从自然老化清漆的薄膜中收集了荧光激发发射光谱。同时，清漆的时间分辨荧光光谱法提供了区分每种复杂材料中短寿命（小于 2.0 ns）和长寿命（大于 7.5 ns）荧光发射的手段。结果表明，两种非破坏性技术的互补使用可以更好地理解导致紫胶发射的主要荧光团，并进一步提供了基于荧光寿命行为差异区分其他清漆主要类别的手段。这里呈现的光谱荧光数据和时间分辨光谱可以为未来对涂漆乐器的原位荧光检查和时间分辨荧光成像结果的解释提供基础。
