Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, Ann Arbor, MI 48109, USA.
Biomaterials. 2009 Dec;30(35):6780-7. doi: 10.1016/j.biomaterials.2009.08.040. Epub 2009 Sep 25.
Cationic magnetic nanoparticles are attractive as potential vehicles for tumor drug delivery due to their favorable interactions with both the tumor milieu and the therapeutic cargo. However, systemic delivery of these nanoparticles to the tumor site is compromised by their rapid plasma clearance. We developed a simple method for in vivo protection of cationic nanocarriers, using non-covalent surface masking with a conjugate of low molecular weight heparin and polyethylene glycol. Surface masking resulted in a 11-fold increase in plasma AUC and a 2-fold increase in the magnetic capture of systemically injected nanoparticles in orthotopic rodent brain tumors. Overall, the described methodology could expand the prospective applications for cationic magnetic nanoparticles in magnetically mediated gene/drug delivery.
阳离子磁性纳米颗粒由于与肿瘤微环境和治疗性载药具有良好的相互作用，因此作为肿瘤药物输送的潜在载体具有吸引力。然而，由于这些纳米颗粒在血浆中迅速清除，因此将它们系统递送至肿瘤部位受到了限制。我们开发了一种简单的体内保护阳离子纳米载体的方法，使用低分子量肝素和聚乙二醇的缀合物进行非共价表面掩蔽。表面掩蔽导致血浆 AUC 增加 11 倍，并且在原位啮齿动物脑肿瘤中系统注射的纳米颗粒的磁捕获增加了 2 倍。总的来说，所描述的方法可以扩展阳离子磁性纳米颗粒在磁性介导的基因/药物传递中的应用前景。
