Laboratório de Enterovírus, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
J Clin Virol. 2009 Dec;46(4):337-40. doi: 10.1016/j.jcv.2009.09.008. Epub 2009 Sep 25.
The virological surveillance of acute flaccid paralysis (AFP) is a critical component of the initiative of the World Health Organization (WHO) to eradicate poliomyelitis worldwide. Furthermore rapid methods are needed either to detect or rule out the presence of polioviruses during the late stages of eradication, especially in polio-free areas.
The aim of this study was to evaluate a fast protocol combining one passage (5 days) in cell culture followed by RT-PCR and molecular typing in order to detect and type poliovirus (PV) and other enteroviruses associated with AFP cases.
A total of 216 fecal suspensions from AFP suspected cases were tested by using this approach and compared with the WHO gold standard.
Using the WHO protocol enterovirus was detected in 12 out of the 216 AFP samples (5.55%) while with the proposed protocol enterovirus was detected in 15 out of the 216 AFP samples (6.94%). The additional positive samples detected by the proposed method were classified as non-polio enteroviruses (NPEV).
The proposed protocol showed higher sensitivity than the WHO gold standard, reducing the entire process of identification and typing of the isolates from the typically 14-21 days to only approximately 6-8 days.
急性弛缓性麻痹（AFP）的病毒学监测是世界卫生组织（WHO）全球根除脊髓灰质炎倡议的一个关键组成部分。此外，在根除的后期阶段，需要快速的方法来检测或排除脊髓灰质炎病毒的存在，尤其是在无脊髓灰质炎地区。
本研究旨在评估一种快速方案，该方案将细胞培养中的一次传代（5 天）与 RT-PCR 和分子分型相结合，以检测和分型与 AFP 病例相关的脊髓灰质炎病毒（PV）和其他肠道病毒。
共对 216 份 AFP 疑似病例的粪便悬液进行了该方法的检测，并与 WHO 金标准进行了比较。
使用 WHO 方案，在 216 份 AFP 样本中有 12 份（5.55%）检测到肠道病毒，而使用所提出的方案，在 216 份 AFP 样本中有 15 份（6.94%）检测到肠道病毒。所提出的方法检测到的额外阳性样本被分类为非脊髓灰质炎肠道病毒（NPEV）。
与 WHO 金标准相比，所提出的方案显示出更高的敏感性，将分离物的鉴定和分型的整个过程从典型的 14-21 天缩短到仅约 6-8 天。
