Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, 17 Changle West Road, Xi'an, 710032, China.
Brain Res. 2009 Dec 22;1304:149-54. doi: 10.1016/j.brainres.2009.09.083. Epub 2009 Sep 25.
HIF-1alpha plays an indispensable role in tumor formation and histogenesis. Target genes involved in glucose transport are acutely transactivated by HIF-1alpha. GLUT-3 protein is the rate-limiting factor related to glucose transport, which is classified as brain-type glucose transporter. This study was the initial one aiming to probe into the co-expression and clinical significance of HIF-1alpha and GLUT-3 in glioma. One hundred and twenty cases of glioma tissues and ten human normal cerebral tissues decompressed in glioma excision were examined using immunohistochemistry and Western blot. The expression of HIF-1alpha and GLUT-3 increased gradually with the increase of pathological grade of glioma, respectively. There was significant difference in the expression of HIF-1alpha and GLUT-3 in every two groups, respectively. There was a positive correlation between HIF-1alpha and GLUT-3. In conclusion, the expression of HIF-1alpha and GLUT-3 in glioma was correlated significantly with tumors' pathological grade, which can be taken as a pair of useful markers for predicting the biological behavior of glioma.
HIF-1alpha 在肿瘤形成和组织发生中起着不可或缺的作用。涉及葡萄糖转运的靶基因被 HIF-1alpha 急性转录激活。GLUT-3 蛋白是与葡萄糖转运相关的限速因子，被归类为脑型葡萄糖转运体。本研究旨在探讨脑胶质瘤中 HIF-1alpha 和 GLUT-3 的共表达及其临床意义。采用免疫组织化学和 Western blot 方法检测 120 例脑胶质瘤组织和 10 例脑胶质瘤切除减压的人正常脑组织中 HIF-1alpha 和 GLUT-3 的表达。HIF-1alpha 和 GLUT-3 的表达随着脑胶质瘤病理分级的增加而逐渐增加。两组之间 HIF-1alpha 和 GLUT-3 的表达均有显著性差异。HIF-1alpha 与 GLUT-3 呈正相关。结论：脑胶质瘤中 HIF-1alpha 和 GLUT-3 的表达与肿瘤的病理分级显著相关，可作为预测脑胶质瘤生物学行为的一对有用标志物。
