Liu Shuyao, Zhong Zhuoling, Zhang Chuanwei, Zhou Yanqu, Fu Chunmei, Xu Xiaoping
West China School of Pharmacy, Sichuan University Chengdu Sichuan 610041 P. R. China
Sichuan Research Center for Drug Precision Industrial Technology, West China School of Pharmacy, Sichuan University Chengdu 610041 China.
Nanoscale Adv. 2021 Dec 17;4(3):894-903. doi: 10.1039/d1na00722j. eCollection 2022 Feb 1.
As a nano-material, carbon dots have been extensively studied and applied in many ways. Herein, iron-doped orange emissive carbon dots (ICDs) were easily synthesized using the hydrothermal method and coupled with Trf and glucose oxidase (GOD) simply by virtue of the abundant functional groups on their surface. The resulting carbon dots were named IGTCDs. The obtained IGTCDs possessed targeting, therapeutic and imaging functions, achieving the enzymolysis of glucose, the decomposition of HO and the release of reactive oxygen species (ROS) sequentially in gliomas as a multifunctional nano-catalyst, and achieving an efficient glioma targeted killing effect. On the basis of the ideal biocompatibility of the IGTCDs with a cell survival rate of over 85%, even at a high concentration (500 μg ml), the IGTCDs, which were coupled substances present within the organism, glucose oxidase and transferrins, showed an obvious inhibitory effect on the growth of tumor cells, and the survival rate of the C6 cells was only 28.10% at 300 μg ml. The highly efficient anti-tumor effect was further demonstrated in the treatment of mice suffering from glioma, and the tumor inhibition rate was increased to 56.21-98.32%. This safe and effective multifunctional tumor inhibitor could be conveniently synthesized in large quantities, verifying the feasibility of the anti-tumor therapy based on the tumor microenvironment (TME), creating a novel method for the application of carbon dots in tumor treatment and providing a novel, reasonable and effective method for the treatment of cancer and gliomas.
作为一种纳米材料，碳点已得到广泛研究并在许多方面得到应用。在此，采用水热法轻松合成了铁掺杂橙色发光碳点（ICDs），并仅凭借其表面丰富的官能团与转铁蛋白（Trf）和葡萄糖氧化酶（GOD）偶联。所得碳点命名为IGTCDs。所制备的IGTCDs具有靶向、治疗和成像功能，作为多功能纳米催化剂在胶质瘤中依次实现葡萄糖的酶解、H₂O₂的分解以及活性氧（ROS）的释放，并实现高效的胶质瘤靶向杀伤作用。基于IGTCDs具有超过85%的细胞存活率这一理想的生物相容性，即使在高浓度（500 μg/ml）下，作为存在于生物体内的偶联物质、葡萄糖氧化酶和转铁蛋白的IGTCDs对肿瘤细胞的生长仍表现出明显的抑制作用，在300 μg/ml时C6细胞的存活率仅为28.10%。在患有胶质瘤的小鼠治疗中进一步证明了其高效的抗肿瘤作用，肿瘤抑制率提高到56.21 - 98.32%。这种安全有效的多功能肿瘤抑制剂能够方便地大量合成，验证了基于肿瘤微环境（TME）的抗肿瘤治疗的可行性，开创了碳点在肿瘤治疗中应用的新方法，并为癌症和胶质瘤的治疗提供了一种新颖、合理且有效的方法。
