Department of Nanomedicine and Biomedical Engineering, The University of Texas Health Science Center Houston, Houston, TX, USA.
Biomaterials. 2010 Jan;31(1):173-9. doi: 10.1016/j.biomaterials.2009.09.018. Epub 2009 Sep 26.
Cellular adhesion is a fundamental process in the development of scaffolds for tissue engineering; in the design of biosensors and in preparing antibacterial substrates. A theoretical model is presented for predicting the strength of cellular adhesion to originally inert surfaces as a function of the substrate topography, accounting for both specific (ligand-receptor) and non-specific interfacial interactions. Three regimes have been identified depending on the surface energy (gamma) of the substrate: for small gamma, any increase in roughness is detrimental to adhesion; for large gamma, an optimal roughness exists that maximizes adhesion; and for intermediate gamma, surface roughness has a minor effect on adhesion. The results presented are in qualitative agreement with several experimental observations and can capture the long-term equilibrium configuration of the system. The model proposed supports the notion for rationally designing substrates where topography and physico-chemical properties are tailored to favour cellular proliferation whilst repelling bacterial adhesion.
细胞黏附是组织工程支架开发、生物传感器设计和制备抗菌基底的基础过程。本文提出了一个理论模型，用于预测细胞黏附到最初惰性表面的强度，该模型考虑了配体-受体特异性和非特异性界面相互作用，作为基底形貌的函数。根据基底表面能 (gamma) ，确定了三种状态：对于小 gamma，任何粗糙度的增加都对黏附有害；对于大 gamma，存在最佳粗糙度，使黏附最大化；对于中等 gamma，表面粗糙度对黏附的影响较小。所提出的结果与一些实验观察结果定性一致，并且可以捕获系统的长期平衡构型。所提出的模型支持合理设计基底的概念，其中形貌和物理化学性质被定制为有利于细胞增殖，同时排斥细菌黏附。
