Department of Biotechnology, Kalasalingam University, Anand Nagar, Krishnankoil 626190, Tamilnadu, India.
Colloids Surf B Biointerfaces. 2010 Jun 1;77(2):257-62. doi: 10.1016/j.colsurfb.2010.02.007. Epub 2010 Feb 11.
The present study demonstrates an unprecedented green process for the production of spherical-shaped Au and Ag nanoparticles synthesized and stabilized using a bacterium, Brevibacterium casei. Aqueous solutions of chloroaurate ions for Au and Ag(+) ions for silver were treated with B. casei biomass for the formation of Au nanoparticles (AuNP) and Ag nanoparticles (AgNP). The nanometallic dispersions were characterized by surface plasmon absorbance measuring at 420 and 540 nm for Ag and Au nanoparticles, respectively. Transmission electron microscopy showed the formation of nanoparticles in the range of 10-50 nm (silver), and 10-50 nm (gold). XRD analysis of the silver and gold nanoparticles confirmed the formation of metallic silver and gold. Further analysis carried out by Fourier Transform Infrared Spectroscopy (FTIR), provides evidence for the presence of proteins as possible biomolecules responsible for the reduction and capping agent which helps in increasing the stability of the synthesized silver and gold nanoparticles. The biological activities of the synthesized particles were confirmed based on their stable anti-coagulant effects. The use of bacterium for nanoparticles synthesis offers the benefits of ecofriendliness and amenability for large-scale production.
本研究展示了一种前所未有的绿色工艺，用于生产使用细菌 Brevibacterium casei 合成和稳定的球形 Au 和 Ag 纳米粒子。用 B. casei 生物量处理氯金酸离子（Au）和银离子（Ag+）的水溶液，以形成 Au 纳米粒子（AuNP）和 Ag 纳米粒子（AgNP）。纳米金属分散体的特征在于在 420nm 和 540nm 处分别测量表面等离子体吸收，以用于 Ag 和 Au 纳米粒子。透射电子显微镜显示在 10-50nm（银）和 10-50nm（金）范围内形成了纳米粒子。银和金纳米粒子的 XRD 分析证实了金属银和金的形成。进一步通过傅里叶变换红外光谱（FTIR）进行的分析提供了证据，证明蛋白质作为可能的生物分子是负责还原和封端剂的物质，这有助于提高合成的银和金纳米粒子的稳定性。根据其稳定的抗凝作用，证实了合成颗粒的生物活性。使用细菌进行纳米粒子合成具有环保和适合大规模生产的优势。
