• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

病毒诱导的基因沉默:一种在植物中发现基因功能的通用工具。

Virus-induced gene silencing: a versatile tool for discovery of gene functions in plants.

机构信息

Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.

出版信息

Plant Physiol Biochem. 2009 Nov-Dec;47(11-12):967-76. doi: 10.1016/j.plaphy.2009.09.001. Epub 2009 Sep 10.

DOI:10.1016/j.plaphy.2009.09.001
PMID:19783452
Abstract

Virus-induced gene silencing (VIGS) is a technology that exploits an antiviral defense mechanism in plants as a tool for plant reverse genetics. VIGS circumvents the need for plant transformation, is methodologically simple and yields rapid results. Various VIGS vectors have been developed and have helped to unravel the functions of genes involved in processes such as disease resistance, abiotic stress, cellular signaling and secondary metabolite biosynthesis.

摘要

病毒诱导的基因沉默(VIGS)是一种利用植物抗病毒防御机制作为植物反向遗传学工具的技术。VIGS 绕过了植物转化的需要,方法简单,结果迅速。已经开发了各种 VIGS 载体,有助于揭示参与疾病抗性、非生物胁迫、细胞信号转导和次生代谢物生物合成等过程的基因的功能。

相似文献

1
Virus-induced gene silencing: a versatile tool for discovery of gene functions in plants.病毒诱导的基因沉默:一种在植物中发现基因功能的通用工具。
Plant Physiol Biochem. 2009 Nov-Dec;47(11-12):967-76. doi: 10.1016/j.plaphy.2009.09.001. Epub 2009 Sep 10.
2
Virus-induced gene silencing for functional analysis of selected genes.用于所选基因功能分析的病毒诱导基因沉默
Plant Cell Rep. 2008 Feb;27(2):209-19. doi: 10.1007/s00299-007-0460-2. Epub 2007 Oct 16.
3
[Virus-induced gene silencing as a tool for analysis of gene functions in plants].病毒诱导的基因沉默作为分析植物基因功能的工具
Uirusu. 2010 Dec;60(2):155-62. doi: 10.2222/jsv.60.155.
4
Virus-induced gene silencing and its application in plant functional genomics.病毒诱导的基因沉默及其在植物功能基因组学中的应用。
Sci China Life Sci. 2012 Feb;55(2):99-108. doi: 10.1007/s11427-012-4280-4. Epub 2012 Mar 15.
5
Virus-induced gene silencing (VIGS) in plants: an overview of target species and the virus-derived vector systems.植物中的病毒诱导基因沉默(VIGS):目标物种及病毒衍生载体系统概述
Methods Mol Biol. 2013;975:1-14. doi: 10.1007/978-1-62703-278-0_1.
6
Virus-induced gene silencing in plants.植物中的病毒诱导基因沉默
Methods. 2003 Aug;30(4):296-303. doi: 10.1016/s1046-2023(03)00037-9.
7
VIGS vectors for gene silencing: many targets, many tools.用于基因沉默的病毒诱导基因沉默载体:众多靶点,多样工具。
Annu Rev Plant Biol. 2004;55:495-519. doi: 10.1146/annurev.arplant.55.031903.141803.
8
The SGN VIGS tool: user-friendly software to design virus-induced gene silencing (VIGS) constructs for functional genomics.SGN VIGS工具:用于功能基因组学的病毒诱导基因沉默(VIGS)构建体设计的用户友好型软件。
Mol Plant. 2015 Mar;8(3):486-8. doi: 10.1016/j.molp.2014.11.024. Epub 2014 Dec 30.
9
Virus-Induced Gene Silencing (VIGS) for Functional Characterization of Disease Resistance Genes in Barley Seedlings.利用病毒诱导基因沉默(VIGS)对大麦幼苗抗病基因进行功能鉴定
Methods Mol Biol. 2019;1900:95-114. doi: 10.1007/978-1-4939-8944-7_7.
10
Virus-induced gene silencing and its application in characterizing genes involved in water-deficit-stress tolerance.病毒诱导的基因沉默及其在鉴定参与水分亏缺胁迫耐受性的基因中的应用。
J Plant Physiol. 2008 Sep 8;165(13):1404-21. doi: 10.1016/j.jplph.2008.04.007. Epub 2008 Jun 9.

引用本文的文献

1
Functional validation of OsRPM1 as a positive regulator of bacterial blight resistance in rice via virus-induced gene silencing.通过病毒诱导的基因沉默对水稻中作为白叶枯病抗性正向调节因子的OsRPM1进行功能验证。
Folia Microbiol (Praha). 2025 Jun 9. doi: 10.1007/s12223-025-01280-6.
2
Silencing of Putative Plasmodesmata-Associated Genes and Reveals Their Differential Involvement during Plant Infection with Cucumber Mosaic Virus.沉默假定的胞间连丝相关基因并揭示其在黄瓜花叶病毒侵染植物过程中的不同作用。
Plants (Basel). 2025 Feb 6;14(3):495. doi: 10.3390/plants14030495.
3
Development of a robust and efficient virus-induced gene silencing system for reverse genetics in recalcitrant Camellia drupifera capsules.
开发一种用于难处理的油茶果核反向遗传学的强大且高效的病毒诱导基因沉默系统。
Plant Methods. 2025 Jan 3;21(1):1. doi: 10.1186/s13007-024-01320-1.
4
Virus-Induced Gene Silencing (VIGS) in and Functional Analysis of .病毒诱导的基因沉默(VIGS)及其功能分析
Plants (Basel). 2024 Dec 3;13(23):3396. doi: 10.3390/plants13233396.
5
Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery.利用谷子花叶病毒介导的sgRNA递送在高粱中进行快速高效的植物基因组编辑。
Plant J. 2025 Jan;121(2):e17196. doi: 10.1111/tpj.17196. Epub 2024 Dec 11.
6
A novel gene silencing strategy based on tobacco rattle virus in .基于烟草脆裂病毒的新型基因沉默策略。
PeerJ. 2024 Oct 7;12:e18211. doi: 10.7717/peerj.18211. eCollection 2024.
7
Down-regulation of wheat Rubisco activase isoforms expression by virus-induced gene silencing.通过病毒诱导的基因沉默下调小麦核酮糖-1,5-二磷酸羧化酶/加氧酶活化酶同工型的表达
Plant Direct. 2024 Apr 15;8(4):e583. doi: 10.1002/pld3.583. eCollection 2024 Apr.
8
Production of Double-Stranded RNA in Planta by a Potato Mop-Top Virus (PMTV)-Based Vector for Inducing Gene Silencing.利用基于马铃薯卷叶病毒(PMTV)的载体在植物中产生双链 RNA 诱导基因沉默。
Methods Mol Biol. 2024;2771:119-126. doi: 10.1007/978-1-0716-3702-9_16.
9
Roles of small RNAs in crop disease resistance.小RNA在作物抗病性中的作用。
Stress Biol. 2021 Aug 18;1(1):6. doi: 10.1007/s44154-021-00005-2.
10
Establishment of virus-induced gene silencing (VIGS) system in Luffa acutangula using Phytoene desaturase (PDS) and tendril synthesis related gene (TEN).利用八氢番茄红素去饱和酶(PDS)和卷须合成相关基因(TEN)在棱角丝瓜中建立病毒诱导的基因沉默(VIGS)系统。
Plant Methods. 2023 Aug 31;19(1):94. doi: 10.1186/s13007-023-01064-4.