Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Department of Genetics, Faculty of Science, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1904-1908. doi: 10.1099/ijs.0.018275-0. Epub 2009 Sep 25.
The taxonomic position of a rhizosphere soil isolate, designated strain SR8.15T, was determined by using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of the strain showed that it formed a well-separated sub-branch within the radiation encompassing the genus Saccharopolyspora. Highest levels of 16S rRNA gene sequence similarity were found between strain SR8.15T and Saccharopolyspora shandongensis CGMCC 4.3530T (98.9%) and Saccharopolyspora spinosa DSM 44228T (98.5%). However, these strains shared low levels of DNA-DNA relatedness (<26%). Strain SR8.15T had chemical characteristics consistent with its classification in the genus Saccharopolyspora. It contained meso-diaminopimelic acid as the diagnostic diamino acid. Whole-cell hydrolysates contained arabinose and galactose. The diagnostic phospholipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The main menaquinone was MK-9(H4). No mycolic acid was detected. The predominant cellular fatty acid was iso-C16:0. The G+C content of the genomic DNA of strain SR8.15T was 70.3 mol%. Strain SR8.15T had a phenotypic profile that readily distinguished it from recognized representatives of the genus Saccharopolyspora. It is evident from its combined genotypic and phenotypic properties that strain SR8.15T represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora phatthalungensis sp. nov. is proposed. The type strain is SR8.15T (=TISTR 1921T=BCC 35844T=NRRL B-24798T).
一株根际土壤分离菌，命名为 SR8.15T 株，采用多相分类法确定其分类地位。基于该菌株近乎完整的 16S rRNA 基因序列的系统发育分析表明，它在包含 Saccharopolyspora 属的辐射范围内形成了一个很好分离的分支。与菌株 SR8.15T 具有最高 16S rRNA 基因序列相似性的是 Saccharopolyspora shandongensis CGMCC 4.3530T（98.9%）和 Saccharopolyspora spinosa DSM 44228T（98.5%）。然而，这些菌株的 DNA-DNA 同源性较低（<26%）。菌株 SR8.15T 的化学特性与其在 Saccharopolyspora 属的分类一致。它含有中 - 二氨基庚二酸作为诊断二氨基酸。全细胞水解物含有阿拉伯糖和半乳糖。诊断磷脂为磷脂酰胆碱、磷脂酰甘油和磷脂酰肌醇。主要的menaquinone 是 MK-9(H4)。未检测到分枝菌酸。主要的细胞脂肪酸是 iso-C16:0。SR8.15T 基因组 DNA 的 G+C 含量为 70.3 mol%。菌株 SR8.15T 的表型特征使其与公认的 Saccharopolyspora 属代表菌株明显区分开来。从其综合表型和遗传特性可以看出，菌株 SR8.15T 代表了 Saccharopolyspora 属的一个新种，建议将其命名为 Phatthalungensis saccharopolyspora sp. nov.。模式菌株为 SR8.15T（=TISTR 1921T=BCC 35844T=NRRL B-24798T）。
