Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, 843300, Xinjiang, People's Republic of China.
Extremophiles. 2011 May;15(3):373-8. doi: 10.1007/s00792-011-0369-0. Epub 2011 Apr 5.
A novel actinomycete strain, designated TRM 40133(T), was isolated from a hypersaline habitat of Tarim basin in Xinjiang Province, north-west China. Its taxonomic status was determined using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of the strain showed that it formed a well-seperated sub-branch within the radiation of the genus Saccharopolyspora. The highest levels of 16S rRNA gene sequence similarity was found between the strain TRM 40133(T) and Saccharopolyspora qijiaojingensis YIM 91168(T) (96.5%). The chemotaxonomic characteristics of the isolate are typical for the genus Saccharopolyspora. It contained meso-DAP as the diagnostic diamino acid. Whole cell hydrolysate contained arabinose, xylose, ribose and glucose. The diagnostic phospholipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol and two unknown phospholipids. The main menaquinone was MK-9(H(6)) and MK-9(H(4)). No mycolic acid was detected. The predominant cellular fatty acids were iso-C(16:0) and anteiso-C(17:0). The G+C content of the genomic DNA was 68.2 mol%. In addition, the strain TRM 40133(T) had a phenotypic profile that readily distinguished it from the recognized representatives of the genus Saccharopolyspora. The strain TRM 40133(T) therefore represents a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora lacisalsi sp. nov. is proposed. The type strain is TRM 40133(T) (=KCTC 19987(T) =CCTCC AA 2010012(T)).
一株新的放线菌菌株，命名为 TRM 40133(T)，从中国西北部新疆塔里木盆地的高盐生境中分离得到。它的分类地位是通过多相方法确定的。基于菌株的近乎完整的 16S rRNA 基因序列的系统发育分析表明，它在糖多孢菌属的辐射中形成了一个很好的分支。该菌株 TRM 40133(T)与 Saccharopolyspora qijiaojingensis YIM 91168(T)（96.5%）的 16S rRNA 基因序列相似度最高。该分离物的化学分类特征是典型的糖多孢菌属。它含有 meso-DAP 作为诊断二氨基庚二酸。全细胞水解物含有阿拉伯糖、木糖、核糖和葡萄糖。诊断性磷脂为磷脂酰胆碱、磷脂酰甘油、磷脂酰肌醇和两种未知磷脂。主要的menaquinone 是 MK-9(H(6)) 和 MK-9(H(4))。未检测到分枝菌酸。主要的细胞脂肪酸是 iso-C(16:0) 和 anteiso-C(17:0)。基因组 DNA 的 G+C 含量为 68.2 mol%。此外，该菌株 TRM 40133(T) 的表型特征很容易将其与糖多孢菌属的公认代表区分开来。因此，该菌株 TRM 40133(T) 代表了糖多孢菌属的一个新种，建议将其命名为拉西盐丝菌（Saccharopolyspora lacisalsi）sp. nov.。模式菌株为 TRM 40133(T)=KCTC 19987(T)=CCTCC AA 2010012(T)。
