Institut für Bakteriologie, Mykologie und Hygiene, Veterinärmedizinische Universität, Veterinärplatz 1, A-1210 Wien, Austria.
Laboratorio de Espiroquetas y Patógenos Especiales, Centro Nacional de Microbiología Instituto de Salud Carlos III, Madrid, Spain.
Int J Syst Evol Microbiol. 2010 Aug;60(Pt 8):1887-1896. doi: 10.1099/ijs.0.015941-0. Epub 2009 Sep 25.
Strain FhSp1T, isolated from human blood in Spain in 2003, was studied for its taxonomic allocation. By 16S rRNA and recA gene sequencing, the strain was shown to belong to the genus Francisella. In the 16S rRNA gene sequence, Francisella sp. FhSp1T shared similarity of more than 99% with strains of Francisella tularensis subspecies and Francisella novicida U112T, 98% with Francisella piscicida GM2212T and 98.4% with Francisella philomiragia ATCC 25015T. In the recA gene sequence, Francisella sp. FhSp1T exhibited 91.6-91.7% similarity to strains of F. tularensis subspecies, 91.2% to F. novicida U112T and 84% to F. philomiragia ATCC 25017. The genus affiliation was supported by a quinone system typical of Francisella (Q-8 as the major component), a complex polar lipid profile similar to that of F. tularensis with the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and an unknown aminophospholipid (APL4) and a fatty acid profile consisting mainly of C10:0 (17.2%), C14:0 (11.2%), C16:0 (13.1%), C18:0 3-OH (14.2%) and C18:1omega9c (7.1%). DNA-DNA hybridization, which showed unambiguously that FhSp1T represents a novel species, and the results of biochemical tests allowed genotypic and phenotypic differentiation of the isolate from all hitherto-described Francisella species. A multiplex PCR developed in the course of this study discriminated FhSp1T from representatives of all other Francisella species and subspecies, clades A.I and A.II of F. tularensis subsp. tularensis and F. tularensis subsp. holarctica biovar japonica and also between these representatives of the genus. Therefore, we propose the name Francisella hispaniensis sp. nov., with the type strain FhSp1T (=FnSp1T =FSC454T =F62T =DSM 22475T =CCUG 58020T). Furthermore, we formally propose the transfer of the species Francisella novicida to the species Francisella tularensis as Francisella tularensis subsp. novicida comb. nov. (type strain ATCC 15482T =CCUG 33449T =CIP 56.12T). We also present an emended description of the genus Francisella.
菌株 FhSp1T 于 2003 年从西班牙的人血中分离出来，对其分类学进行了研究。通过 16S rRNA 和 recA 基因测序，该菌株被归为弗朗西斯菌属。在 16S rRNA 基因序列中，弗朗西斯菌 FhSp1T 与土拉弗朗西斯菌亚种和弗氏新疏螺旋体 U112T 的菌株相似度超过 99%，与鱼类弗朗西斯菌 GM2212T 的相似度为 98%，与嗜肺军团菌 ATCC 25015T 的相似度为 98.4%。在 recA 基因序列中，弗朗西斯菌 FhSp1T 与土拉弗朗西斯菌亚种的菌株相似度为 91.6-91.7%，与弗氏新疏螺旋体 U112T 的相似度为 91.2%，与嗜肺军团菌 ATCC 25017 的相似度为 84%。醌系统（Q-8 为主要成分）典型的弗朗西斯菌属、复杂的极性脂谱与土拉弗朗西斯菌相似，主要成分有双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱和一种未知的氨基磷脂（APL4）以及由 C10:0（17.2%）、C14:0（11.2%）、C16:0（13.1%）、C18:0 3-OH（14.2%）和 C18:1omega9c（7.1%）组成的脂肪酸谱，都支持该菌属于弗朗西斯菌属。DNA-DNA 杂交明确表明 FhSp1T 代表一种新的物种，生化试验的结果使该分离株与所有已描述的弗朗西斯菌物种在基因型和表型上得以区分。在本研究过程中开发的多重 PCR 可将 FhSp1T 与所有其他弗朗西斯菌物种和亚种、土拉弗朗西斯菌亚种 tularensis 的 A.I 和 A.II 分支以及日本亚种的土拉弗朗西斯菌 holarctica 以及该属的其他代表区分开来。因此，我们建议将其命名为弗朗西斯菌 hispaniensis sp. nov.，其模式菌株为 FhSp1T（=FnSp1T =FSC454T =F62T =DSM 22475T =CCUG 58020T）。此外，我们正式提议将弗氏新疏螺旋体转移到土拉弗朗西斯菌中，作为土拉弗朗西斯菌亚种 novicida comb. nov.（模式菌株 ATCC 15482T =CCUG 33449T =CIP 56.12T）。我们还提出了弗朗西斯菌属的修正描述。
