Rusetskaya N V, Lukyanova N Yu, Chekhun V F
R. E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine.
Exp Oncol. 2009 Sep;31(3):140-3.
To compare the molecular profile and cell cycle of sensitive and resistant to doxorubicin MCF-7 breast cancer cells upon exposition to conventional or liposome-encapsulated forms of doxorubicin.
capital EM, Cyrilliccapital TE, Cyrilliccapital TE, Cyrillic-test, immunocytochemistry, flow cytometry.
In sensitive MCF-7 cells the exposure to conventional but not liposomal form of doxorubicin decreased metallothionein (MT) expression demonstrating activation of MT-detoxification system. In doxorubicin-resistant cells (MCF-7/Dsmall o, Cyrillicsmall ha, Cyrillic) MT expression drastically decreased. Conventional but not liposomal form of doxorubicin reduced the levels of expression of steroid hormones receptors on MCF-7 sensitive cells. The exposure of MCF-7 cells to conventional form of doxorubicin resulted in the decrease of p53 expression and the increase of Bcl-2 expression. In MCF-7/Dsmall o, Cyrillicsmall ha, Cyrillic cells neither conventional nor liposomal form of doxorubicin altered Bcl-2 expression. The exposure of MCF-7 but not MCF-7/Dsmall o, Cyrillicsmall ha, Cyrillic to doxorubicin in conventional form caused cell cycle arrest in G0/G1. Upon exposure to doxorubicin in liposomal form, cell cycle blockage in G0/G1 phase was observed in both sensitive and resistant sublines.
The differential effects of the conventional and liposomal forms of doxorubicin in sensitive and resistant cells have been demonstrated. Exposure of MCF-7 and MCF-7/Dsmall o, Cyrillicsmall ha, Cyrillic cells to doxorubicin in liposomal form alters the molecular profile and cell distribution over cell cycle phases.
比较阿霉素敏感和耐药的MCF - 7乳腺癌细胞在暴露于常规或脂质体包裹形式的阿霉素时的分子特征和细胞周期。
大写EM、西里尔大写TE、西里尔大写TE、西里尔测试、免疫细胞化学、流式细胞术。
在敏感的MCF - 7细胞中，暴露于常规而非脂质体形式的阿霉素会降低金属硫蛋白（MT）表达，表明MT解毒系统被激活。在阿霉素耐药细胞（MCF - 7/D小o，西里尔小哈，西里尔）中，MT表达急剧下降。常规而非脂质体形式的阿霉素降低了MCF - 7敏感细胞上类固醇激素受体的表达水平。MCF - 7细胞暴露于常规形式的阿霉素导致p53表达下降和Bcl - 2表达增加。在MCF - 7/D小o，西里尔小哈，西里尔细胞中，常规和脂质体形式的阿霉素均未改变Bcl - 2表达。MCF - 7而非MCF - 7/D小o，西里尔小哈，西里尔暴露于常规形式的阿霉素会导致细胞周期停滞在G0/G1期。当暴露于脂质体形式的阿霉素时，在敏感和耐药亚系中均观察到G0/G1期的细胞周期阻滞。
已证明常规和脂质体形式的阿霉素在敏感和耐药细胞中的不同作用。MCF - 7和MCF - 7/D小o，西里尔小哈，西里尔细胞暴露于脂质体形式的阿霉素会改变分子特征和细胞在细胞周期各阶段的分布。
