Fürstenberg C H, Wiedenhöfer B, Putz C, Burckhardt I, Gantz S, Kleinschmidt K, Schröder K
Querschnittzentrum und Wirbelsäulenchirurgie, Orthopädische Universitätsklinik, Schlierbacher Landstrasse 200a, 69118, Heidelberg, Deutschland.
Orthopade. 2010 Apr;39(4):437-43. doi: 10.1007/s00132-009-1528-1.
The functions of synthetic bone graft substitutes include not only structural support to provide bone healing and osseous ingrowth but also the ability to serve as a local antibiotic delivery system to prevent or treat infections of the spine.
The impregnation and antibiotic efficiency of gentamicin and levofloxacin with Healos was investigated in vitro and compared with Healos without an antibiotic additive. These antibiotic-loaded bone graft substitutes were examined without dilution and with 10-fold and 100-fold dilution for activity against spondylodiscitis-causing bacteria on different agar plates using an agar diffusion method.
All hydroxyapatite (HA)/collagen-saturated diluted antibiotics showed elliptical inhibition zones on the corresponding agar plates. For both antibiotics, there was a linear correlation between dilution and area of the inhibition zone.
The analysis showed that the antimicrobial activity of HA/collagen-saturated antibiotics corresponded to the antimicrobial dilutions. These results should be further analyzed using in vivo studies to determine the remaining antibiotic efficiency after implantation of bone graft substitutes.
合成骨移植替代物的功能不仅包括提供骨愈合和骨长入的结构支撑，还包括作为局部抗生素递送系统以预防或治疗脊柱感染的能力。
在体外研究庆大霉素和左氧氟沙星与Healos的浸渍情况及抗生素效率，并与无抗生素添加剂的Healos进行比较。使用琼脂扩散法，在不同琼脂平板上，对这些负载抗生素的骨移植替代物不稀释以及进行10倍和100倍稀释后，检测其对引起脊椎椎间盘炎的细菌的活性。
所有羟基磷灰石（HA）/胶原饱和的稀释抗生素在相应琼脂平板上均显示出椭圆形抑菌圈。对于这两种抗生素，稀释度与抑菌圈面积之间均存在线性相关性。
分析表明，HA/胶原饱和抗生素的抗菌活性与抗菌稀释度相对应。这些结果应通过体内研究进一步分析，以确定骨移植替代物植入后剩余的抗生素效率。
