Kodaka Hidemasa, Saito Mikako, Matsuoka Hideaki
Nissui Pharmaceutical Co. Ltd., 1075-2, Hokunanmoro, Yuki, Ibaraki 307-0036, Japan.
Biocontrol Sci. 2009 Sep;14(3):123-6. doi: 10.4265/bio.14.123.
The purpose of this study was to evaluate the most-probable-number dilution plate (MPN plate) method developed for the enumeration of Escherichia coil in water samples. Sterilized water was inoculated with E. coli ATCC 11775 to give between 2-1600 MPN/100 ml. The MPN was determined for both the MPN plate and 5-tube methods from the MPN table. The average of the natural logarithm (In) MPN with standard deviations in 95 samples was 4.26 +/- 1.48 by the 5-tube-method and 4.18 +/- 1.45 by the MPN plate method. The correlation coefficient was 0.96. These results were not significantly different according to the paired t-test (p > 0.05).
本研究的目的是评估为测定水样中大肠杆菌而开发的最可能数稀释平板（MPN平板）法。将灭菌水接种大肠杆菌ATCC 11775，使其浓度在2 - 1600 MPN/100 ml之间。根据MPN表分别用MPN平板法和5管法测定MPN。95个样本中，5管法自然对数（In）MPN的平均值及标准差为4.26±1.48，MPN平板法为4.18±1.45。相关系数为0.96。根据配对t检验，这些结果无显著差异（p>0.05）。
