Maizels R M, Page A P
Department of Biology, Imperial College of Science, Technology and Medicine, London, U.K.
Acta Trop. 1990 Jul;47(5-6):355-64. doi: 10.1016/0001-706x(90)90036-y.
The surface of infective larvae of Toxocara canis, the dog ascarid nematode, reveals relatively few exposed surface proteins which can be recovered in soluble form. The major components identified by surface labelling have molecular weights of 32, 55, 70 and 120 kilodaltons (kDa), and are all significantly glycosylated. All are recognised by the immune response in definitive (canine) and paratenic (murine or human) hosts. Expression of these antigens on the parasite surface begins after the larvae hatch from infective ova in vitro, and presumably in vivo. Each of these molecules may also be found in the set of secreted (ES) glycoconjugates released by larval parasites cultivated in vitro, and currently available biochemical and functional data on the surface/secreted ES glycoproteins are presented. Analysis with monoclonal antibodies (MAbs) confirms the identity of surface and ES molecules, and these MAbs show differing patterns of binding to the epicuticle, the cuticular matrix and to the oral orifice. Alternative mechanisms for antigen synthesis, insertion into the cuticle and export from the parasite are discussed.
犬弓首蛔虫（犬类蛔虫线虫）感染性幼虫的表面显示，可溶形式回收的暴露表面蛋白相对较少。通过表面标记鉴定的主要成分的分子量为32、55、70和120千道尔顿（kDa），并且均被显著糖基化。在终末宿主（犬类）和转续宿主（鼠类或人类）中，这些成分均能被免疫反应识别。这些抗原在寄生虫表面的表达在幼虫从感染性虫卵体外孵化后开始，大概在体内也是如此。这些分子中的每一个也可能存在于体外培养的幼虫寄生虫释放的一组分泌性（ES）糖缀合物中，并且本文提供了目前关于表面/分泌性ES糖蛋白的生化和功能数据。用单克隆抗体（MAb）进行分析证实了表面分子和ES分子的一致性，并且这些MAb显示出与表皮、表皮基质以及口腔孔口结合的不同模式。本文还讨论了抗原合成、插入角质层以及从寄生虫中输出的替代机制。
