Dottori Mirella, Pera Martin F
Monash Institute of Medical Research and the Australian Stem Cell Centre, Clayton, Victoria, Australia.
Methods Mol Biol. 2008;438:19-30. doi: 10.1007/978-1-59745-133-8_3.
Embryonic stem cells (ESCs) are pluripotent and capable of indefinite self-renewal in vitro. These features make them a highly advantageous source for deriving any cell type of the central and peripheral nervous system. We describe neural induction of human (h)ESCs, by using the bone morphogenic protein inhibitor protein noggin. Neural progenitors derived from noggin-treated hESCs can be propagated as neurospheres and further differentiated in vitro and in vivo to mature neurons and glia. This complete protocol of neural differentiation, from hESCs to mature neuronal cells, can be used as an in vitro model to study human neurogenesis and neurodegeneration.
胚胎干细胞（ESC）具有多能性，能够在体外无限自我更新。这些特性使其成为衍生中枢和外周神经系统任何细胞类型的极具优势的来源。我们描述了通过使用骨形态发生蛋白抑制剂诺金（noggin）对人（h）胚胎干细胞进行神经诱导。源自经诺金处理的h胚胎干细胞的神经祖细胞可以作为神经球进行增殖，并在体外和体内进一步分化为成熟的神经元和神经胶质细胞。这种从h胚胎干细胞到成熟神经元细胞的完整神经分化方案可作为研究人类神经发生和神经退行性变的体外模型。
