Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.
Food Chem Toxicol. 2010 Jun;48(6):1537-42. doi: 10.1016/j.fct.2010.03.021. Epub 2010 Mar 19.
Antioxidant and antimicrobial activities as well as the quantity of phenolic substances (total phenol, flavonoid and phenolic acid contents) were determined in aqueous extracts of leaves, stems and flowers of Moltkia petraea (Tratt.) Griseb. from two mountainous localities (Sveti Jure and Snijeznica) in Croatia. In addition, the profile of phenolic acids was analyzed by UPLC-MS/MS. Antioxidant activities of all extracts in different test systems, namely the DPPH radical scavenging, reducing power assay and chelating activity, increased with extract concentration. Activity of the extracts from Snijeznica in beta-carotene-linoleic acid assay did not differ from the activity of standard, BHA. The leaf extracts from Snijeznica demonstrated superior antioxidant activity in most of the assays, while the stem extract from the same locality was the most effective Fe(2+) ion chelator. In general, the extracts from Snijeznica were more effective antioxidants than the corresponding extracts from Sveti Jure. The aqueous extracts of M. petraea did not show antimicrobial activity against bacteria and fungi tested in the diffusion and dilution assays.
测定了来自克罗地亚两个山区（Sveti Jure 和 Snijeznica）的 Moltkia petraea（Tratt.）Griseb.的叶、茎和花的水提物的抗氧化和抗菌活性以及酚类物质的含量（总酚、类黄酮和酚酸含量）。此外，还通过 UPLC-MS/MS 分析了酚酸的分布。所有提取物在不同测试系统中的抗氧化活性，即 DPPH 自由基清除能力、还原能力测定和螯合活性，均随提取物浓度的增加而增加。来自 Snijeznica 的提取物在β-胡萝卜素-亚油酸测定中的活性与标准 BHA 的活性没有差异。来自 Snijeznica 的叶提取物在大多数测定中表现出较高的抗氧化活性，而来自同一地点的茎提取物是最有效的 Fe(2+)离子螯合剂。一般来说，来自 Snijeznica 的提取物比来自 Sveti Jure 的相应提取物具有更高的抗氧化活性。水提 M. petraea 提取物在扩散和稀释测定中对测试的细菌和真菌均没有显示出抗菌活性。
