MESA+ Institute for Nanotechnology, University of Twente, P.O. Box 217, 7500 AE Enschede, The Netherlands.
J Magn Reson. 2009 Dec;201(2):175-85. doi: 10.1016/j.jmr.2009.09.007. Epub 2009 Sep 9.
We here report on the optimization, fabrication and experimental characterization of a stripline-based microfluidic NMR probe, realized in a silicon substrate. The stripline geometry was modelled in respect of rf-homogeneity, sensitivity and spectral resolution. Using these models, optimal dimensional ratios were found, which hold for every sample size. Based on the optimized parameters, a simple integrated stripline-based microfluidic chip was realized. The fabrication of this chip is described in detail. We achieved a sensitivity of 0.47 nmol/square root(Hz) and a resolution of 0.7 Hz. The rf-homogeneity (A(810 degrees)/A(90 degrees)) was 76% and was proved to be suitable for 2D-NMR analysis of glucose.
我们在此报告一种基于 stripline 的微流控 NMR 探头的优化、制造和实验特性，该探头是在硅衬底上实现的。 stripline 几何形状在射频均匀性、灵敏度和光谱分辨率方面进行了建模。利用这些模型，找到了最优的尺寸比，这些尺寸比适用于每个样品尺寸。基于优化的参数，实现了一个简单的集成 stripline 微流控芯片。详细描述了该芯片的制造过程。我们实现了 0.47 nmol/√Hz 的灵敏度和 0.7 Hz 的分辨率。射频均匀性（A(810 度)/A(90 度)）为 76%，被证明适用于葡萄糖的 2D-NMR 分析。
