Unidad de Medicina Familiar No 46, Instituto Mexicano del Seguro Social, Monterrey, Mexico.
Acta Diabetol. 2010 Dec;47 Suppl 1:111-6. doi: 10.1007/s00592-009-0149-9. Epub 2009 Sep 29.
We conducted a case-control study to assess whether general DNA damage levels evaluated by comet assay (length of tail comet, tail extent moment, and olive tail moment) differ in Mexican patients with type 2 diabetes mellitus (DM2). The sample included 71 Mexican patients with DM2 who had developed the disease >5 years previously and had been treated with oral hypoglycemic drugs (sulfonylurea and/or metformin), with no microvascular or macrovascular complications. These patients were classified into three groups according to age: (I) 40-50 years, (II) 51-60 years, and (III) 61-70 years. A control group of 14 individuals (40-50 years) without DM2 was included. Our results showed there was a slight but not significant increase in DNA damage in the two groups of patients with DM2 aged between 40 and 60 years compared with the 61-70-year-old patient group and controls. In conclusion, given that general DNA damage was similar in the three groups of patients with DM2 and the control group, it is possible that these cells showed similar oxidative damage, as has been proposed previously.
我们进行了一项病例对照研究，以评估彗星试验（尾部彗星的长度、尾部延伸瞬间和橄榄尾瞬间）评估的一般 DNA 损伤水平是否在患有 2 型糖尿病（DM2）的墨西哥患者中存在差异。该样本包括 71 名患有 DM2 的墨西哥患者，这些患者患有该疾病超过 5 年，并且一直使用口服降糖药物（磺酰脲类和/或二甲双胍）治疗，没有微血管或大血管并发症。这些患者根据年龄分为三组：（I）40-50 岁，（II）51-60 岁，（III）61-70 岁。还包括了一个年龄在 40-50 岁之间、没有 DM2 的 14 人对照组。我们的结果表明，与 61-70 岁的患者组和对照组相比，40-60 岁的两组 DM2 患者的 DNA 损伤略有但无统计学意义的增加。总之，鉴于三组 DM2 患者和对照组的一般 DNA 损伤相似，这些细胞可能表现出类似的氧化损伤，正如之前提出的那样。
