Binici D N, Karaman A, Coşkun M, Oğlu A Uyanik, Uçar F
Department of Internal Medicine, Erzurum Training and Research Hospital, Erzurum, Turkey.
Genet Couns. 2013;24(2):149-56.
DNA damage seems to play a role in the pathogenesis of type-2 diabetes mellitus (DM2) and its complications. Several in vitro assays have been used to measure the DNA damage. In the present study, we aimed to investigate the frequency of sister chromatid exchange (SCE) and micronuclei (MN) in DM2 patients compared with healthy controls. SCE and MN tests were carried out with the blood-cell cultures from 50 DM2 patients and 30 healthy, age- and sex-matched control subjects. The mean age of the DM2 patients was 58.12 +/- 13.39 years, with a mean duration of the diabetes of 5.40 +/- 4.32 years. The mean level of HbAlc of the DM2 patients was 8.93 +/- 2.56. Patients with DM2 showed a higher frequency of SCE compared with controls (7.11 +/- 1.14 and 4.96 +/- 0.92, p < 0.001). Furthermore, the SCE frequency was positively correlated with the plasma HbA1c level (p < 0.05), but there was no significant correlation between the duration of diabetes and SCE. On the other hand, our result showed a MN frequency significant increase in DM2 patients (3.45 +/- 1.01 per 1000 cells) relative to that of the control group (1.79 +/- 0.67 per 1000 cells) (p < 0.001), but there was no significant correlation between the duration of diabetes, HbA1c and MN. In conclusion, these results suggest that DM2 is a condition with genomic instability characterized by an increased level of SCE and MN. Hyperglycemia-induced oxidative stress may be the underlying factor of the increased SCE and MN frequency.
DNA损伤似乎在2型糖尿病（DM2）及其并发症的发病机制中起作用。已经使用了几种体外试验来测量DNA损伤。在本研究中，我们旨在调查与健康对照相比，DM2患者的姐妹染色单体交换（SCE）和微核（MN）频率。对50例DM2患者和30例年龄和性别匹配的健康对照受试者的血细胞培养物进行SCE和MN检测。DM2患者的平均年龄为58.12±13.39岁，糖尿病平均病程为5.40±4.32年。DM2患者的平均糖化血红蛋白（HbAlc）水平为8.93±2.56。与对照组相比，DM2患者的SCE频率更高（分别为7.11±1.14和4.96±0.92，p<0.001）。此外，SCE频率与血浆HbA1c水平呈正相关（p<0.05），但糖尿病病程与SCE之间无显著相关性。另一方面，我们的结果显示，相对于对照组（每1000个细胞中1.79±0.67个），DM2患者的MN频率显著增加（每1000个细胞中3.45±1.01个）（p<0.001），但糖尿病病程、HbA1c与MN之间无显著相关性。总之，这些结果表明，DM2是一种具有基因组不稳定的疾病，其特征是SCE和MN水平升高。高血糖诱导的氧化应激可能是SCE和MN频率增加的潜在因素。
