Sexton J L, Milner A R, Panaccio M, Waddington J, Wijffels G, Chandler D, Thompson C, Wilson L, Spithill T W, Mitchell G F
Veterinary Research Institute, Department of Agriculture and Rural Affairs, Attwood, Australia.
J Immunol. 1990 Dec 1;145(11):3905-10.
The potential of GST as a vaccine candidate against liver fluke infection in ruminants was studied by vaccinating sheep (n = 9) with GST purified from adult worms of Fasciola hepatica and challenging with 500 F. hepatica metacercariae. The immunization induced a high antibody response to GST in contrast to the poor or undetectable response to this Ag observed in naturally infected sheep. Throughout the trial, the progress of the fluke infection was monitored by measuring RBC hemoglobin levels, the extent of liver damage and the fecal egg output in the sheep. This analysis indicated that a subpopulation (n = 4) of the GST vaccinated animals exhibited no anemia, reduced liver damage and a lower mean fecal egg count relative to the infected control group suggesting a lower fluke burden in these animals. Worm burdens in the livers of the GST vaccine group (107 +/- 22) were 57% lower than in the infected control group (250 +/- 25). The subpopulation of the GST vaccine group demonstrated a 78% reduction in mean worm burdens relative to the control group. These results show that GST of adult F. hepatica is a novel Ag that can significantly protect sheep against liver fluke infection. The results suggest that the immune response to GST is directed to the juvenile worm reducing the number of worms that can establish in the liver of the vaccinated animals.
通过用从肝片吸虫成虫中纯化的谷胱甘肽 S-转移酶（GST）对绵羊（n = 9）进行免疫接种，并以 500 个肝片吸虫囊蚴进行攻毒，研究了 GST 作为反刍动物抗肝吸虫感染候选疫苗的潜力。与自然感染绵羊中观察到的对该抗原的微弱或无法检测到的反应相比，免疫接种诱导了对 GST 的高抗体反应。在整个试验过程中，通过测量绵羊的红细胞血红蛋白水平、肝损伤程度和粪便虫卵产量来监测吸虫感染的进展。该分析表明，与感染对照组相比，GST 免疫接种动物的一个亚群（n = 4）没有出现贫血，肝损伤减轻，平均粪便虫卵计数较低，这表明这些动物体内的吸虫负担较低。GST 疫苗组肝脏中的虫体负担（107 +/- 22）比感染对照组（250 +/- 25）低 57%。GST 疫苗组的亚群相对于对照组平均虫体负担减少了 78%。这些结果表明，肝片吸虫成虫的 GST 是一种新型抗原，可显著保护绵羊免受肝吸虫感染。结果表明，对 GST 的免疫反应针对幼虫，减少了可在接种动物肝脏中定植的虫体数量。
