Laboratory of Pathophysiology, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
PLoS One. 2009 Sep 30;4(9):e7247. doi: 10.1371/journal.pone.0007247.
It has been shown that molecular hydrogen (H(2)) acts as a therapeutic antioxidant and suppresses brain injury by buffering the effects of oxidative stress. Chronic oxidative stress causes neurodegenerative diseases such as Parkinson's disease (PD). Here, we show that drinking H(2)-containing water significantly reduced the loss of dopaminergic neurons in PD model mice using both acute and chronic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). The concentration-dependency of H(2) showed that H(2) as low as 0.08 ppm had almost the same effect as saturated H(2) water (1.5 ppm). MPTP-induced accumulation of cellular 8-oxoguanine (8-oxoG), a marker of DNA damage, and 4-hydroxynonenal (4-HNE), a marker of lipid peroxidation were significantly decreased in the nigro-striatal dopaminergic pathway in mice drinking H(2)-containing water, whereas production of superoxide (O(2)*(-)) detected by intravascular injection of dihydroethidium (DHE) was not reduced significantly. Our results indicated that low concentration of H(2) in drinking water can reduce oxidative stress in the brain. Thus, drinking H(2)-containing water may be useful in daily life to prevent or minimize the risk of life style-related oxidative stress and neurodegeneration.
已经证明，氢气（H₂）作为一种治疗性抗氧化剂，通过缓冲氧化应激的影响来抑制脑损伤。慢性氧化应激会导致帕金森病（PD）等神经退行性疾病。在这里，我们通过急性和慢性给予 1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP），显示饮用含氢水可显著减少 PD 模型小鼠多巴胺能神经元的丢失。H₂的浓度依赖性表明，即使 H₂的浓度低至 0.08 ppm，也几乎与饱和 H₂水（1.5 ppm）具有相同的效果。饮用含氢水的小鼠黑质纹状体多巴胺能通路中，MPTP 诱导的细胞 8-氧鸟嘌呤（8-oxoG，DNA 损伤的标志物）和 4-羟基壬烯醛（4-HNE，脂质过氧化的标志物）的积累明显减少，而通过血管内注射二氢乙啶（DHE）检测到的超氧阴离子（O₂*（-））的产生并未显著减少。我们的结果表明，饮用水中低浓度的 H₂可以减轻大脑中的氧化应激。因此，饮用含氢水可能在日常生活中有用，可以预防或最小化与生活方式相关的氧化应激和神经退行性变的风险。
