Han Ki-Ho, Han Song-I, Frazier A Bruno
School of Nano Engineering, Inje University, Obang-dong, Gimhae, GyongNam, 621-749, Republic of Korea.
Lab Chip. 2009 Oct 21;9(20):2958-64. doi: 10.1039/b909753h. Epub 2009 Aug 5.
We describe the lateral displacement of a particle passing over a planar interdigitated electrode array at an angle as a function of the particle size. The lateral displacement was also measured as a function of the angle between the electrode and the direction of flow. A simplified line charge model was used for numerically estimating the lateral displacement of fluorescent polystyrene (PS) beads with three different diameters. Using the lateral displacement as a function of particle size, we developed a lateral dielectrophoretic (DEP) microseparator, which enables continuous discrimination of particles by size. The microchannel was divided into three regions, each with an electrode array placed at a different angle with respect to the direction of flow. The experiment using an admixture of 3-, 5-, and 10-microm PS beads showed that the lateral DEP microseparator could continuously separate out 99.86% of the 3-microm beads, 98.82% of the 5-microm beads, and 99.69% of the 10-microm beads, simply by using a 200-kHz 12-Vp-p AC voltage to create the lateral DEP force. The lateral DEP microseparator is thus a practical device for simultaneously separating particles according to size from a heterogeneous admixture.
我们描述了以一定角度通过平面叉指电极阵列的粒子的横向位移与粒径的函数关系。横向位移也作为电极与流动方向之间夹角的函数进行了测量。使用简化的线电荷模型对三种不同直径的荧光聚苯乙烯(PS)微珠的横向位移进行了数值估算。利用横向位移与粒径的函数关系,我们开发了一种横向介电泳(DEP)微分离器,它能够按粒径对粒子进行连续区分。微通道被分为三个区域,每个区域都有一个相对于流动方向以不同角度放置的电极阵列。使用3微米、5微米和10微米PS微珠的混合物进行的实验表明,横向DEP微分离器仅通过施加200kHz、12Vp-p的交流电压来产生横向DEP力,就能连续分离出99.86%的3微米微珠、98.82%的5微米微珠和99.69%的10微米微珠。因此,横向DEP微分离器是一种实用的装置,可用于从异质混合物中按粒径同时分离粒子。