大豆中的限制性片段长度多态性（RFLP）图谱构建：标记位点与数量性状变异之间的关联
RFLP mapping in soybean: association between marker loci and variation in quantitative traits.
作者信息
Keim P, Diers B W, Olson T C, Shoemaker R C
机构信息
Department of Agronomy, Iowa State University, Ames 50011.
出版信息
Genetics. 1990 Nov;126(3):735-42. doi: 10.1093/genetics/126.3.735.
Abstract
We have constructed a genetic map for soybean and identified associations between genetic markers and quantitative trait loci. One-hundred-fifty restriction fragment length polymorphisms (RFLPs) were used to identify genetic linkages in an F2 segregating population from an interspecific cross (Glycine max x Glycine soja). Twenty-six genetic linkage groups containing ca. 1200 recombination units are reported. Progeny-testing of F2-derived families allowed quantitative traits to be evaluated in replicated field trials. Genomic regions, which accounted for a portion of the genetic variation (R2 = 16 to 24%) in several reproductive and morphological traits, were linked to RFLP markers. Significant associations between RFLP markers and quantitative trait loci were detected for eight of nine traits evaluated. The ability to identify genes within a continuously varying trait has important consequences for plant breeding and for understanding evolutionary processes.
摘要
我们构建了大豆遗传图谱，并确定了遗传标记与数量性状基因座之间的关联。利用150个限制性片段长度多态性（RFLP）来鉴定种间杂交（大豆×野生大豆）F2分离群体中的遗传连锁。报道了包含约1200个重组单位的26个遗传连锁群。对F2衍生家系进行后代测试，以便在重复的田间试验中评估数量性状。基因组区域与RFLP标记连锁，这些区域在几个生殖和形态性状中占一部分遗传变异（R2 = 16%至24%）。在所评估的九个性状中的八个中检测到RFLP标记与数量性状基因座之间存在显著关联。在连续变化的性状中鉴定基因的能力对植物育种和理解进化过程具有重要意义。
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