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本文引用的文献

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Identification of the genomic locations of duplicate nucleotide sequences in maize by analysis of restriction fragment length polymorphisms.通过分析限制性片段长度多态性鉴定玉米中重复核苷酸序列的基因组位置。
Genetics. 1988 Feb;118(2):353-63. doi: 10.1093/genetics/118.2.353.
2
Haploid selection for low temperature tolerance of tomato pollen.利用单倍体选择提高番茄花粉的耐低温性。
Genetics. 1982 May;101(1):129-37. doi: 10.1093/genetics/101.1.129.
3
Use of monosomics to map cloned DNA fragments in maize.利用单体型鉴定将克隆 DNA 片段定位在玉米中。
Proc Natl Acad Sci U S A. 1986 Aug;83(16):6035-9. doi: 10.1073/pnas.83.16.6035.
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The molecular genetics of human hemoglobins.人类血红蛋白的分子遗传学。
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5
MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations.MAPMAKER：一个用于构建实验群体和自然群体初级遗传连锁图谱的交互式计算机程序包。
Genomics. 1987 Oct;1(2):174-81. doi: 10.1016/0888-7543(87)90010-3.
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Molecular-marker-facilitated investigations of quantitative-trait loci in maize. I. Numbers, genomic distribution and types of gene action.分子标记辅助的玉米数量性状基因座研究。I. 数量、基因组分布及基因作用类型
Genetics. 1987 May;116(1):113-25. doi: 10.1093/genetics/116.1.113.
7
Organization of the Tgm family of transposable elements in soybean.大豆中转座元件Tgm家族的组织方式
Genetics. 1988 Oct;120(2):597-604. doi: 10.1093/genetics/120.2.597.
8
Mapping mendelian factors underlying quantitative traits using RFLP linkage maps.利用限制性片段长度多态性连锁图谱定位数量性状的孟德尔因子。
Genetics. 1989 Jan;121(1):185-99. doi: 10.1093/genetics/121.1.185.

大豆中的限制性片段长度多态性（RFLP）图谱构建：标记位点与数量性状变异之间的关联

RFLP mapping in soybean: association between marker loci and variation in quantitative traits.

作者信息

Keim P, Diers B W, Olson T C, Shoemaker R C

机构信息

Department of Agronomy, Iowa State University, Ames 50011.

出版信息

Genetics. 1990 Nov;126(3):735-42. doi: 10.1093/genetics/126.3.735.

DOI:10.1093/genetics/126.3.735
PMID:1979039
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1204227/
Abstract

We have constructed a genetic map for soybean and identified associations between genetic markers and quantitative trait loci. One-hundred-fifty restriction fragment length polymorphisms (RFLPs) were used to identify genetic linkages in an F2 segregating population from an interspecific cross (Glycine max x Glycine soja). Twenty-six genetic linkage groups containing ca. 1200 recombination units are reported. Progeny-testing of F2-derived families allowed quantitative traits to be evaluated in replicated field trials. Genomic regions, which accounted for a portion of the genetic variation (R2 = 16 to 24%) in several reproductive and morphological traits, were linked to RFLP markers. Significant associations between RFLP markers and quantitative trait loci were detected for eight of nine traits evaluated. The ability to identify genes within a continuously varying trait has important consequences for plant breeding and for understanding evolutionary processes.

摘要

我们构建了大豆遗传图谱，并确定了遗传标记与数量性状基因座之间的关联。利用150个限制性片段长度多态性（RFLP）来鉴定种间杂交（大豆×野生大豆）F2分离群体中的遗传连锁。报道了包含约1200个重组单位的26个遗传连锁群。对F2衍生家系进行后代测试，以便在重复的田间试验中评估数量性状。基因组区域与RFLP标记连锁，这些区域在几个生殖和形态性状中占一部分遗传变异（R2 = 16%至24%）。在所评估的九个性状中的八个中检测到RFLP标记与数量性状基因座之间存在显著关联。在连续变化的性状中鉴定基因的能力对植物育种和理解进化过程具有重要意义。